Frequency of allele loss of DCC, p53, RBI, WT1, NF1, NM23 and APC/MCC in colorectal cancer assayed by fluorescent multiplex polymerase chain reaction.
Frequency of allele loss of DCC, p53, RBI, WT1, NF1, NM23 and APC/MCC in colorectal cancer assayed by fluorescent multiplex polymerase chain reaction.
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DOI：
10.1038/bjc.1994.404
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发表时间：
1994-11
影响因子：
8.8
通讯作者：
Quirke, P.
中科院分区：
文献类型：
--
作者：
Cawkwell, L.;Lewis, F. A.;Quirke, P.
我们在这里报告使用多重荧光聚合酶链反应（PCR）的定量等位基因丢失检测使用微卫星与2-5个碱基对重复基序。先前已经使用多种方法报道了结直肠肿瘤中APC、DCC、p53和RB 1的等位基因丢失。然而，并不是所有的工人使用基因内标记。我们已经使用微卫星多态性的地图内，或紧密相连，这些肿瘤抑制基因位点，以确定这些位点是否确实是改变结直肠癌的目标。此外，我们还检测了另外两个肿瘤抑制基因WT 1和NF 1，以了解它们是否在结直肠癌发生中发挥作用。假定的转移抑制基因，NM 23，也进行了研究，因为有相互矛盾的报告，其参与结直肠癌的发生。在DCC（29%）、p53（66%）、RB 1（50%）和NF 1（14%）位点以及APC/MCC区域（50%）检测到等位基因丢失，但在WT 1或NM 23位点未检测到等位基因丢失。这些快速的，大多是基因特异性的，荧光多重PCR检测等位基因丢失检测可以修改设计一个单一的分子诊断测试的重要病变在结直肠癌。
We report here the use of multiplex fluorescent polymerase chain reaction (PCR) for quantitative allele loss detection using microsatellites with 2-5 base pair repeat motifs. Allele loss of APC, DCC, p53 and RB1 in colorectal tumours has been reported previously using a variety of methods. However, not all workers used intragenic markers. We have used microsatellite polymorphisms which map within, or are closely linked to, these tumour-suppressor gene loci in order to determine whether these loci are indeed the targets for alteration in colorectal cancer. In addition, we have assayed two other tumour-suppressor genes, WT1 and NF1, to see whether they play a role in colorectal carcinogenesis. The putative metastasis-suppressor gene, NM23, was also investigated since there have been conflicting reports about its involvement in colorectal carcinogenesis. Allele loss was detected at the DCC (29%), p53 (66%), RB1 (50%) and NF1 (14%) loci and in the APC/MCC region (50%), but not at the WT1 or NM23 loci. These rapid, and mostly gene-specific, fluorescent multiplex PCR assays for allele loss detection could be modified to devise a single molecular diagnostic test for the important lesions in colorectal cancer.
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影响因子：
64.5
作者：
LI, Y;BOLLAG, G;CAWTHON, R
通讯作者：
CAWTHON, R
影响因子：
8.8
作者：
Meling, G I;Lothe, R A;Borresen, A L;Hauge, S;Graue, C;Clausen, O P;Rognum, T O
通讯作者：
Rognum, T O
DOI：
10.1093/jnci/85.2.147
发表时间：
1993-01-20
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
MYEROFF, LL;MARKOWITZ, SD
通讯作者：
MARKOWITZ, SD
影响因子：
3.5
作者：
HAUGE, XY;LITT, M
通讯作者：
LITT, M
影响因子：
8.8
作者：
Cawkwell, L;Bell, S M;Lewis, F A;Dixon, M F;Taylor, G R;Quirke, P
通讯作者：
Quirke, P