Poly(C)-Binding Protein 1, a Novel Npro-Interacting Protein Involved in Classical Swine Fever Virus Growth
Poly(C)-Binding Protein 1, a Novel Npro-Interacting Protein Involved in Classical Swine Fever Virus Growth
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Poly(C)-结合蛋白 1，一种参与猪瘟病毒生长的新型 Npro 相互作用蛋白
DOI：
10.1128/jvi.02807-12
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发表时间：
2013-02-01
影响因子：
5.4
通讯作者：
Qiu, Hua-Ji
中科院分区：
文献类型：
--
作者：
Li, Dan;Li, Su;Qiu, Hua-Ji
Npro是瘟病毒特有的一种多功能自身蛋白酶。猪瘟病毒（classical swine fever virus，CSFV）的Npro蛋白的相互作用伴侣尚未完全确定。使用酵母双杂交筛选，我们确定聚（C）-结合蛋白1（PCBP 1）作为一种新的相互作用的合作伙伴的猪瘟病毒Npro蛋白，并证实了共免疫沉淀，谷胱甘肽S-转移酶（GST）下拉，共聚焦检测。敲低PCBP 1的小干扰RNA抑制CSFV的生长，而过表达PCBP 1促进CSFV的生长。此外，我们发现I型干扰素被PCBP 1以及Npro下调。我们的研究结果表明，细胞PCBP 1积极调节猪瘟病毒的生长。
ABSTRACT Npro is a multifunctional autoprotease unique to pestiviruses. The interacting partners of the Npro protein of classical swine fever virus (CSFV), a swine pestivirus, have been insufficiently defined. Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV Npro protein and confirmed this by coimmunoprecipitation, glutathione S-transferase (GST) pulldown, and confocal assays. Knockdown of PCBP1 by small interfering RNA suppressed CSFV growth, while overexpression of PCBP1 promoted CSFV growth. Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as Npro. Our results suggest that cellular PCBP1 positively modulates CSFV growth.