IDENTIFICATION OF THE INTEGRIN VLA-2 AS A RECEPTOR FOR ECHOVIRUS-1
IDENTIFICATION OF THE INTEGRIN VLA-2 AS A RECEPTOR FOR ECHOVIRUS-1
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DOI：
10.1126/science.1553561
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发表时间：
1992-03-27
期刊：
影响因子：
56.9
通讯作者：
FINBERG, RW
中科院分区：
文献类型：
--
作者：
BERGELSON, JM;SHEPLEY, MP;FINBERG, RW
ECHO病毒是一种常见的人类病原体，其细胞表面受体是用保护易感细胞免受ECHO病毒1型感染的单抗鉴定的。这些单抗阻止病毒与特定的受体结合，识别整合素VLA-2的α和β亚基(α-2-β-1)，整合素α-2-β-1是胶原和层粘连蛋白的受体。RD横纹肌肉瘤细胞表达少量的VLA-2，不与S-35标记的病毒结合，直到转染编码VLA-2α-2亚单位的互补DNA后才能抵抗感染。因此，整合素是一种黏附受体，在细胞之间和与细胞外基质的相互作用中起重要作用，可以介导病毒附着和感染。
Cell surface receptors for echovirus, a common human pathogen, were identified with monoclonal antibodies that protected susceptible cells from infection with echovirus 1. These monoclonal antibodies, which prevented virus attachment to specific receptor sites, recognized the alpha and beta-subunits of the integrin VLA-2 (alpha-2-beta-1), a receptor for collagen and laminin. RD rhabdomyosarcoma cells expressed little VLA-2, did not bind to S-35-labeled virus, and resisted infection until transfected with complementary DNA encoding the alpha-2 subunit of VLA-2. Thus, integrins, adhesion receptors important in interactions between cells and with the extracellular matrix, can mediate virus attachment and infection.