Entamoeba histolytica cytotoxin: purification, characterization, strain virulence, and protease activity.
Entamoeba histolytica cytotoxin: purification, characterization, strain virulence, and protease activity.
复制标题
溶组织内阿米巴细胞毒素：纯化、表征、菌株毒力和蛋白酶活性。
DOI：
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发表时间：
1982
影响因子：
6.4
通讯作者：
S. Gorbach
中科院分区：
文献类型：
--
作者：
K. McGowan;C. Deneke;G. Thorne;S. Gorbach
从无菌培养的溶组织内阿米巴（Entamoeba histolytica）强毒株中分离到一种不耐热细胞毒素。株大肠代表在动物和体外疾病模型中测定毒力谱的溶组织菌的细胞毒活性。强毒株HM 1的提取物具有明显的细胞毒活性，中毒株200的提取物具有中等活性，无毒毒株303和Rahman的提取物没有活性。从超声处理的E.通过硫酸铵沉淀和凝胶过滤分离溶组织菌HM 1滋养体。细胞毒性活性在窄pH范围（6-7.2）和1 M NaCl、尿素和胍中稳定。特异性免疫兔和人抗血清以及蛋白酶抑制剂抑肽酶、胃酶抑素和亮抑酶肽抑制细胞毒性。部分纯化的细胞毒素没有任何可检测的降解酶活性。因此，E.溶组织阿米巴具有免疫原性细胞毒性蛋白，其在阿米巴病的病理生理学中可能是重要的。
A heat-labile cytotoxin was isolated from virulent strains of axenically cultivated Entamoeba histolytica. Strains of E. histolytica representing a spectrum of virulence as determined in animal and in vitro models of disease were examined for cytotoxic activity. Extracts of virulent strain HM1 possessed marked cytotoxic activity, those of moderately virulent strain 200 showed intermediate activity, and those of avirulent strains 303 and Rahman showed no activity. The cytotoxin was partially purified from the cell-free supernatant of sonicated E. histolytica HM1 trophozoites by ammonium sulfate precipitation and gel filtration. Cytotoxic activity was stable in a narrow pH range (6-7.2) and in 1 M NaCl, urea, and guanidine. Specific immune rabbit and human antiserum as well as the protease inhibitors aprotinin, pepstatin, and leupeptin inhibited cytotoxicity. The partially purified cytotoxin did not have any detectable degradative enzymatic activities. Thus, virulent strains of E. histolytica possess an immunogenic cytotoxic protein which may be important in the pathophysiology of amoebiasis.
α1 抗蛋白酶和 α2 巨球蛋白抑制溶组织内阿米巴细胞毒素。
DOI：
10.4269/ajtmh.1981.30.575
发表时间：
1981
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Lushbaugh,WB;Kairalla,AB;Hofbauer,AF;Arnaud,P;Cantey,JR;Pittman,FE
通讯作者：
Pittman,FE