Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation
Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation
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DOI：
10.1016/j.jbiotec.2004.03.006
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发表时间：
2004-07-01
影响因子：
4.1
通讯作者：
Deckwer, WD
中科院分区：
文献类型：
--
作者：
Hollmann, R;Deckwer, WD
一个重组巨大芽孢杆菌菌株表现出分泌大量丙酮酸的能力（高达27.8 g l（-1））的生长速率大于0.15 h（-1）。低于该生长速率的培养避免了丙酮酸的形成，同时使乙酸和琥珀酸的产生最小化。使用指数进料，最终生物质浓度高达80克升（-1）实现。丙酮酸形成实验的总摩尔产率高达0.79 mol mol（-1）。丙酮酸形成是由糖酵解和丙酮酸脱氢酶反应/三羧酸循环能力之间的差异在葡萄糖过量。高丙酮酸导致生长减速和随后的停止。此外，这种抑制作用可能与B使用的磷酸烯醇丙酮酸：葡萄糖磷酸转移酶系统有关。作为葡萄糖的主要输入者。(C)2004 Elsevier B. V.保留所有权利。
A recombinant Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8 g l(-1)) for growth rates larger than 0.15 h(-1). Cultivation below this growth rate avoids pyruvate formation while minimizing acetate and succinate production. Using exponential feeding, final biomass concentrations of up to 80 g l(-1) were achieved. Overall molar yields for the experiments with pyruvate formation were as high as 0.79 mol mol(-1). Pyruvate formation was caused by the discrepancy between glycolytic and pyruvate dehydrogenase reaction/tricarboxylic acid cycle capacities during glucose excess. High pyruvate resulted in deceleration and subsequent cessation of growth. In addition, this inhibitory effect is likely associated with the phoshoenolpyruvate:glucose phosphotransferase system used by B. megaterium as the main importer for glucose. (C) 2004 Elsevier B.V. All rights reserved.