Plasma Metabolomics Analysis Based on GC-MS in Infertile Males with Kidney-Yang Deficiency Syndrome.
Plasma Metabolomics Analysis Based on GC-MS in Infertile Males with Kidney-Yang Deficiency Syndrome.
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基于GC-MS的肾阳虚证不育男性血浆代谢组学分析
DOI：
10.1155/2017/6270195
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Luo J
中科院分区：
文献类型：
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作者：
Zheng P;Wang Y;Lu H;Zhou X;Tang T;Fan R;Zhang C;Cui H;Wang Y;Luo J
前言中医证候诊断是中医药治疗男性不育症的关键。肾阳虚证是男性不育的重要中医证候。本研究对男性不育症患者的代谢组学进行了研究，旨在探讨KYDS的现代化机制。方法采用气相色谱-质谱联用法对67例男性不育症患者和55例健康对照者的血浆进行分析。化学计量学方法，包括主成分和偏最小二乘判别分析，以确定潜在的生化模式。在投影和受试者工作特征曲线分析的变量重要性的帮助下，提取潜在的生物标志物以定义临床效用。同时利用高质量的KEGG代谢途径数据库对相关代谢途径进行了识别。结果确定了KYDS患者的代谢组学特征，包括10个潜在的生物标志物和6条代谢途径。他们精确地区分了患有KYDS的不育男性和健康对照。结论这些潜在的生物标志物和通路为KYDS男性不育提供了物质基础。代谢组学特征突出了KYDS不育男性的现代化机制。
Introduction Chinese medicine syndrome diagnosis is the key requisite in the treatment of male infertility with traditional Chinese medicine (TCM). Kidney-Yang deficiency syndrome (KYDS) is the critical Chinese medicine syndrome of male infertility. To explore the modernized mechanisms of KYDS in male infertility, this study aims to investigate the metabolomics of males with KYDS. Methods The gas chromatography-mass spectrometry method was applied to analyze the plasma samples of 67 infertile males with KYDS compared with 55 age-matched healthy controls. The chemometric methods including principal component and partial least squares-discriminate analyses were employed to identify the potential biochemical patterns. With the help of the variable importance for the projection and receiver operating characteristic curve analyses, the potential biomarkers were extracted to define the clinical utility. Simultaneously the high-quality KEGG metabolic pathways database was used to identify the related metabolic pathways. Results The metabolomics profiles of infertile males with KYDS including 10 potential biomarkers and six metabolic pathways were identified. They precisely distinguished infertile males with KYDS from healthy controls. Conclusions These potential biomarkers and pathways suggest the substantial basis of infertile males with KYDS. The metabolomics profiles highlight the modernized mechanisms of infertile males with KYDS.