Expression of epiregulin, a novel epidermal growth factor ligand associated with prognosis in human oral squamous cell carcinomas.
Expression of epiregulin, a novel epidermal growth factor ligand associated with prognosis in human oral squamous cell carcinomas.
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DOI：
10.3892/or.19.6.1557
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发表时间：
2008-06
期刊：
影响因子：
4.2
通讯作者：
H. Shigeishi;K. Higashikawa;Misato Hiraoka;S. Fujimoto;Y. Mitani;K. Ohta;M. Takechi;N. Kamata
中科院分区：
文献类型：
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作者：
H. Shigeishi;K. Higashikawa;Misato Hiraoka;S. Fujimoto;Y. Mitani;K. Ohta;M. Takechi;N. Kamata
我们采用实时荧光定量pcr检测了39例口腔SCCs、2例上皮发育不良和7例正常牙龈中表调节蛋白和双调节蛋白mRNA的表达。表调节蛋白mRNA在口腔SCCs中的平均表达水平（0.29+/-0.50）高于正常牙龈（0.01+/-0.007）和上皮发育不良（0.01+/-0.001）。表调节蛋白mRNA在口腔SCCs中的表达水平显著高于正常牙龈（Mann-Whitney U检验，P=0.023）。表调节蛋白mRNA在III/IV期口腔SCCs中高于I/II期。然而，没有发现显著的关联。双调节蛋白mRNA在口腔SCCs中的平均表达量（0.18+/-0.24）高于正常牙龈（0.002+/-0.003）和上皮发育不良（0.01+/-0.001）。双侧调节蛋白mRNA在口腔SCCs中的表达明显高于正常牙龈（Mann-Whitney U检验，P=0.001）。然后我们检测了四种EGF受体mRNA在口腔SCCs中的表达。口腔口腔SCCs中HER1、HER2、HER3和HER4 mRNA的表达水平均高于正常牙龈。表调节蛋白mRNA表达水平与HER2、HER3、HER4有显著相关性（Spearman相关系数秩检验，P=0.031、P=0.004、P=0.027）。表调节蛋白高表达的口腔鳞状细胞癌患者的生存期明显短于低表达的患者（log-rank检验，P<0.05）。这些结果表明，人表调节蛋白与人口腔鳞状细胞癌细胞增殖增加或异常密切相关。
We examined the expression of epiregulin and amphiregulin mRNA in 39 oral SCCs, 2 epithelial dysplasias and 7 normal gingivae by real-time RT-PCR. The mean expression level of epiregulin mRNA was higher in oral SCCs (0.29+/-0.50) than normal gingivae (0.01+/-0.007) and epithelial dysplasias (0.01+/-0.001). The expression level of epiregulin mRNA was significantly higher in oral SCCs than normal gingivae (Mann-Whitney U test, P=0.023). Epiregulin mRNA was higher in stage III/IV than in stage I/II oral SCCs. However, a significant association was not found. The mean expression level of amphiregulin mRNA was higher in oral SCCs (0.18+/-0.24) than normal gingivae (0.002+/-0.003) and epithelial dysplasias (0.01+/-0.001). Amphiregulin mRNA was significantly higher in oral SCCs than normal gingivae (Mann-Whitney U test, P=0.001). We then examined the expression of four EGF receptor mRNA in oral SCCs. The expression levels of HER1, HER2, HER3 and HER4 mRNA in oral oral SCCs were increased compared to those in normal gingivae. A significant correlation was found between the mRNA expression levels of epiregulin and HER2, HER3 and HER4 (Spearman's correlation coefficient by rank test, P=0.031, P=0.004 and P=0.027, respectively). Patients with oral SCC that have a high expression of epiregulin had a significantly shorter survival than those with low a expression (log-rank test, P<0.05). These results indicate that human epiregulin is closely linked to the increased or abnormal cell proliferation in human oral SCC.