Immunohistochemical Study of Protein 4.1B in the Normal and W/Wv Mouse Seminiferous Epithelium
Immunohistochemical Study of Protein 4.1B in the Normal and W/Wv Mouse Seminiferous Epithelium
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DOI：
10.1369/jhc.3a6192.2004
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发表时间：
2004-06
影响因子：
3.2
通讯作者：
N. Terada;N. Ohno;H. Yamakawa;T. Baba;Y. Fujii;Zagreb Zea;O. Ohara;S. Ohno
中科院分区：
文献类型：
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作者：
N. Terada;N. Ohno;H. Yamakawa;T. Baba;Y. Fujii;Zagreb Zea;O. Ohara;S. Ohno
细胞-细胞粘附不仅对于机械粘附而且对于组织形态发生是至关重要的。蛋白4.1B是蛋白4.1家族的成员，来源于红细胞膜蛋白，是粘附系统的潜在组织者。在成年小鼠曲细精管中，蛋白4.1B定位于基底室，尤其是精原细胞和支持细胞的附着区。4.1B蛋白在各生精期的定位和表达无明显差异。在发育过程中，蛋白4.1B在出生后第3天（P3）未被检测到，在P15时被弥散定位，并在第三周期间在基底室中被发现。通过蛋白4.1B和F-肌动蛋白的双重染色，它们的定位被证明是不同的，表明蛋白4.1B定位在低于形成Sertoli-Sertoli连接的基底内质网特化的区域。电镜下免疫反应产物主要分布于支持细胞膜上。在W/Wv突变小鼠中，生精上皮具有很少的生殖细胞。未检测到蛋白4.1B和β-连环蛋白，但保留了基础的内质网特化。提示4.1B蛋白可能与支持细胞与生殖细胞尤其是精原细胞的粘附有关。
Cell-cell adhesion is crucial not only for mechanical adhesion but also for tissue morphogenesis. Protein 4.1B, a member of the protein 4.1 family named from an erythrocyte membrane protein, is a potential organizer of an adherens system. In adult mouse seminiferous tubules, protein 4.1B localized in the basal compartment, especially in the attaching region of spermatogonia and Sertoli cells. Protein 4.1B localization and appearance were not different in each spermatogenic stage. Developmentally, protein 4.1B was not detected at postnatal day 3 (P3), was diffusely localized at P15, and was found in the basal compartment during the third week. By double staining for protein 4.1B and F-actin, their localizations were shown to be different, indicating that protein 4.1B was localized in a region lower than the basal ectoplasmic specialization that formed the Sertoli–Sertoli junction. By electron microscopy, immunoreactive products were seen mainly on the membranes of Sertoli cells. In the W/Wv mutant mouse, the seminiferous epithelium had few germ cells. Protein 4.1B and β-catenin were not detected, although the basal ectoplasmic specialization was retained. These results indicate that protein 4.1B may be related to the adhesion between Sertoli cells and germ cells, especially the spermatogonium.