Flagellin from an incompatible strain of Acidovorax avenae mediates H2O2 generation accompanying hypersensitive cell death and expression of PAL, Cht-1, and PBZ1, but not of Lox in rice.
Flagellin from an incompatible strain of Acidovorax avenae mediates H2O2 generation accompanying hypersensitive cell death and expression of PAL, Cht-1, and PBZ1, but not of Lox in rice.
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DOI：
10.1094/mpmi.2003.16.5.422
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发表时间：
2003-05
期刊：
影响因子：
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通讯作者：
N. Tanaka;F. Che;N. Watanabe;S. Fujiwara;S. Takayama;A. Isogai
中科院分区：
文献类型：
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作者：
N. Tanaka;F. Che;N. Watanabe;S. Fujiwara;S. Takayama;A. Isogai
燕麦食酸菌引起单子叶植物的褐色条纹病。我们最近报道了一个水稻不亲和的A.麦长管蚜引起水稻过敏性细胞死亡，不亲和菌株的鞭毛蛋白参与了这种反应。不亲和菌株诱导H2 O2的快速产生，伴随着过敏性细胞死亡和防御基因的表达，如PAL，Cht-1，PBZ 1，和LOX，而兼容菌株没有。纯化的不亲和鞭毛蛋白还诱导PAL、Cht-1和PBZ 1的表达，但不诱导LOX的表达。接种不相容菌株后，PAL和LOX酶活性增加，而不相容鞭毛蛋白仅增加PAL酶活性。有趣的是，鞭毛蛋白缺陷型不亲和菌株失去了产生H2 O2和诱导过敏性细胞死亡的能力，但PAL，Cht-1和PBZ 1的表达仍然被诱导接种缺陷型菌株，这表明这些基因的诱导不仅受鞭毛蛋白，但也受到一些其他信号。因此，A.麦长管蚜是水稻中的特异性激发子，但它不是诱导水稻防御系统的唯一因子。
Acidovorax avenae causes a brown stripe disease in monocot plants. We recently reported that a rice-incompatible strain of A. avenae caused hypersensitive cell death in rice and that the flagellin of the incompatible strain was involved in this response. The incompatible strain induced the rapid generation of H2O2 accompanying hypersensitive cell death and the expression of defense genes such as PAL, Cht-1, PBZ1, and LOX, whereas the compatible strain did not. The purified incompatible flagellin also induced the expression of PAL, Cht-1, and PBZ1, but LOX expression was not induced by the incompatible flagellin. PAL and LOX enzymatic activities were increased by inoculation with the incompatible strain, whereas only PAL activity was increased by the incompatible flagellin. Interestingly, the flagellin-deficient incompatible strain lost the ability to generate H2O2 and induce hypersensitive cell death, but PAL, Cht-1, and PBZ1 expression still were induced by inoculation with the deficient strain, suggesting that induction of these genes is regulated not only by flagellin but also by some other signal. Thus, the incompatible flagellin of A. avenae is a specific elicitor in rice, but it is not the only factor capable of inducing the rice defense system.