Maternal control of seed weight in rapeseed (Brassica napus L.): the causal link between the size of pod (mother, source) and seed (offspring, sink)
Maternal control of seed weight in rapeseed (Brassica napus L.): the causal link between the size of pod (mother, source) and seed (offspring, sink)
复制标题
油菜种子重量的母体控制（Brassica napus L.）：豆荚大小（母亲，源）和种子（后代，库）之间的因果关系
DOI：
10.1111/pbi.13011
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发表时间：
2019-04-01
影响因子：
13.8
通讯作者：
Wang, Hanzhong
中科院分区：
文献类型：
--
作者：
Li, Na;Song, Ongji;Wang, Hanzhong
摘要 种子大小/重量是与植物驯化和作物改良相关的关键性状之一。在油菜籽（Brassica napus L.）种质中，种子重量表现出广泛的变异，但其调节机制知之甚少。为了确定种子重量调节的关键机制，进行了系统的比较研究。遗传、形态学和细胞学证据表明，种子重量受母本基因型控制，主要通过细胞数量调节种子大小。生理证据表明，荚果长度的差异可能导致荚果壁光合面积、碳水化合物和最终种子重量的差异。我们还鉴定了两个多效性主要数量性状基因座，它们通过对豆荚长度的影响而间接影响种子重量。 RNA-seq结果表明，与荚果发育和激素相关的基因在荚果壁中表达存在显着差异；与发育、细胞分裂、营养库和核糖体蛋白相关的基因在大型种子库的种子中均上调。最后，我们提出了一种潜在的种子重量调节机制，该机制是油菜籽特有且在植物中新颖的。结果证明了油菜籽荚的大小（母亲，源）和种子（后代，库）之间的因果关系，这为母体控制种子重量提供了新的见解，并将开辟植物的新研究领域。
Summary Seed size/weight is one of the key traits related to plant domestication and crop improvement. In rapeseed (Brassica napus L.) germplasm, seed weight shows extensive variation, but its regulatory mechanism is poorly understood. To identify the key mechanism of seed weight regulation, a systematic comparative study was performed. Genetic, morphological and cytological evidence showed that seed weight was controlled by maternal genotype, through the regulation of seed size mainly via cell number. The physiological evidence indicated that differences in the pod length might result in differences in pod wall photosynthetic area, carbohydrates and the final seed weight. We also identified two pleiotropic major quantitative trait loci that acted indirectly on seed weight via their effects on pod length. RNA‐seq results showed that genes related to pod development and hormones were significantly differentially expressed in the pod wall; genes related to development, cell division, nutrient reservoir and ribosomal proteins were all up‐regulated in the seeds of the large‐seed pool. Finally, we proposed a potential seed weight regulatory mechanism that is specific to rapeseed and novel in plants. The results demonstrate a causal link between the size of the pod (mother, source) and the seed (offspring, sink) in rapeseed, which provides novel insight into the maternal control of seed weight and will open a new research field in plants.