Chronic electromyograms in treadmill running SOD1 mice reveal early changes in muscle activation.
Chronic electromyograms in treadmill running SOD1 mice reveal early changes in muscle activation.
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在跑步机上跑步的 SOD1 小鼠的慢性肌电图揭示了肌肉激活的早期变化。
DOI：
10.1113/jp274170
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Tysseling,VickiM
中科院分区：
文献类型：
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作者：
Quinlan,KatharinaA;Kajtaz,Elma;Ciolino,JodyD;Imhoff-Manuel,RebeccaD;Tresch,MatthewC;Heckman,CharlesJ;Tysseling,VickiM
关键点本研究表明，在小鼠运动活动过程中获得的肌电图（EMG）可有效识别肌萎缩侧索硬化（ALS）的早期生理标志物。这些措施可用于评估ALS动物模型中的治疗干预策略。SOD 1G 93 A小鼠在疾病时间过程的早期，特别是当跑步机倾斜时，本研究的结果表明，早期的代偿性变化可能发生在神经网络内控制运动活动，包括脊髓interneurons.运动肌电图可能有潜在的用途作为一个临床诊断tool.AbstractTo提高我们对早期疾病机制的理解，并确定可靠的生物标志物的肌萎缩侧索硬化症（ALS），进行性神经退行性疾病，我们测量了肌电图（EMG）活动在后肢肌肉的SOD 1G 93 A小鼠。通过对比临床诊断措施，使用肌电图，这是对静止的病人，我们监测活动在跑步机上运行，旨在检测运动模式的症状前变化。慢性肌电图电极植入股外侧肌，股二头肌后，腓肠肌外侧和胫骨前的小鼠从出生后55至100天，并获得的结果进行了评估，使用线性混合模型。我们评估了当动物在水平和倾斜的跑步机上跑步时，与EMG振幅（峰值和面积）和时间（相位和偏斜，突发形状的测量）相关的参数的差异。SOD 1G 93 A小鼠的活动时间和肌电图爆发的幅度都有显着变化。野生型和SOD 1G 93 A小鼠之间的显著差异主要是在动物在倾斜的旋转磨上旋转时观察到的。所有的肌肉都有显著的突变效应，与年龄无关。这些新的结果表明：（i）运动EMG活动可能是疾病发作的早期测量;（ii）运动模式的改变可能反映了网络水平上神经元驱动和补偿的变化，包括脊髓中间神经元的活动改变;（iii）倾斜跑步机上所需的功率输出增加对于揭示SOD 1G 93 A小鼠活动改变很重要。
Key pointsThe present study demonstrates that electromyograms (EMGs) obtained during locomotor activity in mice were effective for identification of early physiological markers of amyotrophic lateral sclerosis (ALS). These measures could be used to evaluate therapeutic intervention strategies in animal models of ALS.Several parameters of locomotor activity were shifted early in the disease time course in SOD1G93A mice, especially when the treadmill was inclined, including intermuscular phase, burst skew and amplitude of the locomotor bursts.The results of the present study indicate that early compensatory changes may be taking place within the neural network controlling locomotor activity, including spinal interneurons.Locomotor EMGs could have potential use as a clinical diagnostic tool.AbstractTo improve our understanding of early disease mechanisms and to identify reliable biomarkers of amyotrophic lateral sclerosis (ALS), a progressive neurodegenerative disease, we measured electromyogram (EMG) activity in hind limb muscles of SOD1G93A mice. By contrast to clinical diagnostic measures using EMGs, which are performed on quiescent patients, we monitored activity during treadmill running aiming to detect presymptomatic changes in motor patterning. Chronic EMG electrodes were implanted into vastus lateralis, biceps femoris posterior, lateral gastrocnemius and tibialis anterior in mice from postnatal day 55 to 100 and the results obtained were assessed using linear mixed models. We evaluated differences in parameters related to EMG amplitude (peak and area) and timing (phase and skew, a measure of burst shape) when animals ran on level and inclined treadmills. There were significant changes in both the timing of activity and the amplitude of EMG bursts in SOD1G93A mice. Significant differences between wild‐type and SOD1G93A mice were mainly observed when animals locomoted on inclined treadmills. All muscles had significant effects of mutation that were independent of age. These novel results indicate (i) locomotor EMG activity might be an early measure of disease onset; (ii) alterations in locomotor patterning may reflect changes in neuronal drive and compensation at the network level including altered activity of spinal interneurons; and (iii) the increased power output necessary on an inclined treadmill was important in revealing altered activity in SOD1G93A mice.