1H NMR visibility of mammalian glycogen in solution.
1H NMR visibility of mammalian glycogen in solution.
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溶液中哺乳动物糖原的 1H NMR 可见度。
DOI：
10.1073/pnas.87.5.1678
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发表时间：
1990
影响因子：
11.1
通讯作者：
Shulman,RG
中科院分区：
文献类型：
--
作者：
Zang,LH;Rothman,DL;Shulman,RG
在500 MHz下获得了兔肝糖原在2 H2O中的高分辨率1H NMR谱，并通过与α-连接的二葡萄糖分子的化学位移的比较来分配几个共振。糖原在2 H2O中的NMR弛豫时间T1和T2被确定为分别为1.1和0.029 s。测得的自然线宽的碳-1质子（12 +/- 2 Hz）是在极好的协议与计算从T2。通过消化糖原并比较葡萄糖和糖原信号强度进行的可见度测量表明，尽管分子量非常高，但糖原中的所有质子核都对NMR光谱有贡献。该结果并不意外，因为由于快速的分子内运动，先前从糖原的碳核观察到100%NMR可见性。
High-resolution 1H NMR spectra of rabbit liver glycogen in 2H2O were obtained at 500 MHz, and several resonances were assigned by comparison with the chemical shifts of alpha-linked diglucose molecules. The NMR relaxation times T1 and T2 of glycogen in 2H2O were determined to be 1.1 and 0.029 s, respectively. The measured natural linewidth of the carbon-1 proton (12 +/- 2 Hz) is in excellent agreement with that calculated from T2. The visibility measurements made by digesting glycogen and comparing glucose and glycogen signal intensities demonstrate that in spite of the very high molecular weight, all of the proton nuclei in glycogen contribute to the NMR spectrum. The result is not unexpected, since 100% NMR visibility was previously observed from the carbon nuclei of glycogen, due to the rapid intramolecular motions.