A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells
A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells
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DOI：
10.1016/j.biomaterials.2004.03.005
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发表时间：
2005-02-01
期刊：
影响因子：
14
通讯作者：
Tuan, RS
中科院分区：
文献类型：
--
作者：
Li, WJ;Tuli, R;Tuan, RS
在组织工程中利用成体干细胞是解决组织或器官短缺问题的一个有前景的方法。成人骨髓来源的间充质干细胞（MSCs）是未分化的多能细胞，当在三维培养环境中培养，并使用转化生长因子 -β（TGF -β）家族的生长因子进行处理时，能够分化为软骨细胞。在这项研究中，我们制作了一种由可生物降解的合成聚合物聚己内酯（PCL）制成的纳米纤维支架（NFS），并检测了其支持MSCs体外软骨形成的能力。电纺PCL多孔支架由直径为700nm的均匀、随机取向的纳米纤维构成，并且在21天的培养期内该支架保持了结构完整性。在含有TGF -β1的NFS中培养的MSCs分化为软骨细胞表型，这通过软骨细胞特异性基因表达以及软骨相关细胞外基质（ECM）蛋白的合成得以证明。在接种于NFS内的MSCs中观察到的软骨形成水平与作为细胞聚集体或团块培养的MSCs所观察到的水平相当，细胞聚集体或团块是一种广泛用于研究MSCs体外软骨形成的培养方案。由于NFS的物理性质和改进的机械性能，特别是与细胞团块相比，此处报道的研究结果表明PCL NFS是MSCs移植的一种实用载体，并且是基于细胞的组织工程方法用于软骨修复的一种候选支架。（C）2004爱思唯尔有限公司。保留所有权利。
The utilization of adult stem cells in tissue engineering is a promising solution to the problem of tissue or organ shortage. Adult bone marrow derived mesenchymal stem cells (MSCs) are undifferentiated, multipotential cells which are capable of giving rise to chondrocytes when maintained in a three-diniensional culture and treated with members of the transforming growth factor-beta (TGF-beta) family of growth factors. In this study, we fabricated a nanofibrous scaffold (NFS) made of a synthetic biodegradable polymer, poly(epsilon-caprolactoile) (PCL), and examined its ability to support in vitro chondrogenesis of MSCs. The electrospun PCL porous scaffold was constructed of uniform, randomly oriented nanofibers with a diameter of 700 nm, and structural integrity of this scaffold was maintained over a 21-day culture period. MSCs cultured in NFSs in the presence of TGF-beta1 differentiated to a chondrocytic phenotype, as evidenced by chondrocyte-specific gene expression and synthesis of cartilage-associated extracellular matrix (ECM) proteins. The level of chondrogenesis observed in MSCs seeded within NFSs was comparable to that observed for MSCs maintained as cell aggregates or pellets, a widely used culture protocol for studying chondrogenesis of MSCs in vitro. Due to the physical nature and improved mechanical properties of NFSs, particularly in comparison to cell pellets, the findings reported here suggest that the PCL NFS is a practical carrier for MSC transplantation, and represents a candidate scaffold for cell-based tissue engineering approaches to cartilage repair. (C) 2004 Elsevier Ltd. All rights reserved.