Isolation of segmented filamentous bacteria from complex gut microbiota
Isolation of segmented filamentous bacteria from complex gut microbiota
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DOI：
10.2144/000114319
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发表时间：
2015-08-01
期刊：
影响因子：
2.7
通讯作者：
Franklin, Craig L.
中科院分区：
文献类型：
--
作者：
Ericsson, Aaron C.;Turner, Giedre;Franklin, Craig L.
分节丝状细菌（SFB）调节免疫系统的个体发育，它们的存在可以显着影响疾病的小鼠模型。直到最近，由于无法成功培养SFB，对这些细菌发挥影响的机制进行了对照研究。在这里，我们报告了一种从复杂的微生物混合物中选择SFB的新方法，为研究人员提供了一种简单且具有成本效益的方法来制备纯感染性接种物用于前瞻性研究，并比较单个SFB分离株。
Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates.