A novel long noncoding RNA, ENSGALG00000021686, regulates the intracellular transport of fatty acids by targeting the FABP3 gene in chicken
A novel long noncoding RNA, ENSGALG00000021686, regulates the intracellular transport of fatty acids by targeting the FABP3 gene in chicken
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一种新型长非编码 RNA ENSGALG00000021686 通过靶向鸡的 FABP3 基因来调节脂肪酸的细胞内转运
DOI：
10.1016/j.bbrc.2020.05.133
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发表时间：
2020-08-06
影响因子：
3.1
通讯作者：
Chang, Guobin
中科院分区：
文献类型：
--
作者：
Bi, Yulin;Yuan, Xiaoya;Chang, Guobin
脂肪酸（FAs）是人类和动物重要运动所必需的。它们的代谢部分由FABP 3调节。在我们之前的研究中，发现了一种新的lncRNA（ENSGALG 00000021686，L21686），并预测FABP 3是其靶基因。本研究以鸡的肌细胞、淋巴细胞和不同组织为靶点，探讨L21686对FABP 3基因、FABP 3 mRNA表达及其对FA代谢的影响。结果表明，L21686在肌肉组织中的表达最高，肌肉组织是一个显著的能量消耗组织。L21686表达与FABP 3 mRNA表达一致。我们还发现，在不同的处理下，FABP 3 mRNA和蛋白在心肌细胞和淋巴细胞中的水平与L21686的表达同步变化。此外，双荧光素酶报告基因试验提供了L21686靶向FABP 3基因的直接证据。最后，发现游离脂肪酸的含量随着L21686和FABP 3基因的上调而沿着增加。丙二酰辅酶A含量在不同处理下没有变化，表明L21686调节鸡细胞外脂肪酸的摄入。此外，脂蛋白脂酶（LPL）、固醇调节元件结合蛋白1（SREBP-1）、脂肪酸合成酶（FATIGUE）和乙酰辅酶A羧化酶（ACC）mRNA水平的变化支持了这一观点。总之，我们的数据表明，新的lncRNA（L21686）通过靶向FABP 3基因的表达，在体外调节鸡细胞中细胞外FA的摄入。本研究结果将为揭示鸡脂肪酸代谢的调控机制奠定基础并提供新的线索。(C)2020爱思唯尔公司All rights reserved.
Fatty acids (FAs) are essential for the vital movement of humans and animals. Their metabolism is, in part, regulated by FABP3. In our previous study, a novel lncRNA (ENSGALG00000021686, L21686) was identified, and FABP3 was predicted as its target gene. Here, using chicken myocytes, lymphocytes, and different tissues, L21686 target on the FABP3 gene, FABP3 mRNA expression, and their effect on FA metabolism are explored. The results show that the highest expression of L21686 is in muscle tissue, a significant energy-consuming tissue. L21686 expression is consistent with FABP3 mRNA expression. We also show that under the different treatments, the levels of FABP3 mRNA and protein in myocytes and lymphocytes change in tandem with L21686 expression. Moreover, the dual-luciferase reporter assay provided direct evidence that L21686 targets the FABP3 gene. Finally, it was found that the content of free FAs increases along with the up-regulation of L21686 and the FABP3 gene. Malonyl CoA content does not change under the different treatments, suggesting that L21686 regulates the intake of extracellular FAs in chicken. Further, the changes in lipoprotein lipase (LPL), sterol-regulatory element binding protein 1 (SREBP-1), fatty acid synthase (FASN), and acetyl-CoA carboxylase (ACC) mRNA levels support this view. In summary, our data show that the new lncRNA (L21686) regulates the intake of extracellular FAs in chicken cells in vitro by targeting the expression of the FABP3 gene. Our findings will help to establish the groundwork and provide a new clue for deciphering the regulation of FAs metabolism in chicken. (C) 2020 Elsevier Inc. All rights reserved.