Extended guidelines for mtDNA typing of population data in forensic science
Extended guidelines for mtDNA typing of population data in forensic science
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DOI：
10.1016/j.fsigen.2006.11.003
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发表时间：
2007-03-01
影响因子：
3.1
通讯作者：
Bandelt, Hans-Juergen
中科院分区：
文献类型：
--
作者：
Parson, Walther;Bandelt, Hans-Juergen
线粒体DNA分析已成为法医科学的一个重要领域，因为它构成了低质量和低数量DNA样本的强大技术。在法医领域，重要的是采用基于科学依据的标准化程序，以便法庭接受mtDNA证据。在这里，我们修改和扩展了之前在缺乏关于全球系统发育的坚实知识的情况下阐明的建议。在样品选择、扩增和测序策略以及mtDNA谱的事后质量控制方面，有必要改进这些早期指南。因此，序列数据的符号应该反映这种增长的知识。2006爱思唯尔爱尔兰有限公司版权所有。
Mitochondrial DNA analysis has become a vital niche in forensic science as it constitutes a powerful technique for low quality and low quantity DNA samples. For the forensic field it is important to employ standardized procedures based on scientific grounds, in order to have mtDNA evidence be accepted in court. Here, we modify and extend recommendations that were spelled out previously in the absence of solid knowledge about the worldwide phylogeny. Refinement of those earlier guidelines became necessary in regard to sample selection, amplification and sequencing strategies, as well as a posteriori quality control of mtDNA profiles. The notation of sequence data should thus reflect this growing knowledge. (C) 2006 Elsevier Ireland Ltd. All rights reserved.