Increased Reactive Oxygen Species Formation and Oxidative Stress in Rheumatoid Arthritis.
Increased Reactive Oxygen Species Formation and Oxidative Stress in Rheumatoid Arthritis.
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DOI：
10.1371/journal.pone.0152925
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Fatima N
中科院分区：
文献类型：
--
作者：
Mateen S;Moin S;Khan AQ;Zafar A;Fatima N
风湿性关节炎（RA）是一种自身免疫性炎症性疾病。高活性氧自由基被认为参与疾病的发病机制。在这项研究中，RA患者根据是否存在类风湿因子、疾病活动评分和疾病持续时间进行亚组。RA患者（120例）和健康对照（53例）通过监测ROS产生、脂质过氧化、蛋白质氧化和DNA损伤的生物标志物来评估氧化-抗氧化状态。还监测了各种酶和非酶抗氧化剂的水平。还进行了相关性分析，以分析ROS与各种其他参数之间的关联。检测RA患者外周血细胞内ROS生成、脂质过氧化（MDA水平）、蛋白质氧化（羰基水平和巯基水平）和DNA损伤。抗氧化状态通过FRAP测定、DPPH还原测定以及酶（SOD、过氧化氢酶、GST、GR）和非酶（维生素C和GSH）抗氧化剂来评估。RA患者体内活性氧产生增多，脂质过氧化、蛋白质氧化和DNA损伤增加。还观察到铁还原能力、DPPH自由基淬灭能力和抗氧化剂水平的显著下降。已发现ROS和其他各种参数之间的显着相关性研究。RA患者表现出ROS形成、脂质过氧化、蛋白质氧化、DNA损伤和抗氧化防御系统活性降低的显著增加，导致氧化应激，这可能有助于组织损伤，从而导致疾病的慢性化。
Rheumatoid arthritis (RA) is an autoimmune inflammatory disorder. Highly reactive oxygen free radicals are believed to be involved in the pathogenesis of the disease. In this study, RA patients were sub-grouped depending upon the presence or absence of rheumatoid factor, disease activity score and disease duration. RA Patients (120) and healthy controls (53) were evaluated for the oxidant—antioxidant status by monitoring ROS production, biomarkers of lipid peroxidation, protein oxidation and DNA damage. The level of various enzymatic and non-enzymatic antioxidants was also monitored. Correlation analysis was also performed for analysing the association between ROS and various other parameters. Intracellular ROS formation, lipid peroxidation (MDA level), protein oxidation (carbonyl level and thiol level) and DNA damage were detected in the blood of RA patients. Antioxidant status was evaluated by FRAP assay, DPPH reduction assay and enzymatic (SOD, catalase, GST, GR) and non-enzymatic (vitamin C and GSH) antioxidants. RA patients showed a higher ROS production, increased lipid peroxidation, protein oxidation and DNA damage. A significant decline in the ferric reducing ability, DPPH radical quenching ability and the levels of antioxidants has also been observed. Significant correlation has been found between ROS and various other parameters studied. RA patients showed a marked increase in ROS formation, lipid peroxidation, protein oxidation, DNA damage and decrease in the activity of antioxidant defence system leading to oxidative stress which may contribute to tissue damage and hence to the chronicity of the disease.