Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)
Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)
复制标题
提取微囊藻毒素在杂食性鲫鱼肾脏的蓄积及腹腔注射的影响
DOI：
10.1016/j.toxicon.2013.03.022
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发表时间：
2013-08-01
期刊：
影响因子：
2.8
通讯作者：
Zhang, Xuezhen
中科院分区：
文献类型：
--
作者：
Li, Li;Xie, Ping;Zhang, Xuezhen
采用急性毒性实验研究了鲫鱼腹腔注射（i. p.）与提取的微囊藻毒素（主要是MC-RR和-LR）在两个剂量，50和200 μ g MC-LReq。kg（-1）体重。分别采用HPLC和LC-MS对肾脏中MC进行定量和定性测定。鲫鱼肾脏中MC-RR含量在注射后48 h内呈时间-剂量依赖性增加，随后急剧下降，而在整个实验过程中未检测到MC-LR。鲫鱼肾脏超微结构的变化在48 h内随着MCs累积量的增加和暴露时间的延长而增加，而在50 μ g MC-LReq中鲫鱼肾脏超微结构的恢复。kg（-1）剂量组在注射后168 h明显。我们的超微结构观察表明，膜结构是MCs在肾脏中的主要作用部位，其中肾小管中的线粒体损伤显然是MCs的早期且可能是至关重要的作用。两个剂量组血尿素氮（BUN）和肌酐（CR）的升高进一步表明鲫鱼肾脏发生严重损伤。(C)2013爱思唯尔有限公司保留所有权利。
An acute toxicological experiment was designed to characterize the sequence of renal ultrastructural changes with accumulated MCs in crucian carp injected intraperitoneally (i.p.) with extracted microcystins (mainly MC-RR and -LR) at two doses, 50 and 200 mu g MC-LReq. kg(-1) body weight. Quantitative and qualitative determinations of MCs in the kidney were conducted by HPLC and LC-MS, respectively. MC-RR content in kidney of crucian carp showed a time dose-dependent increase within 48 h post-injection, followed by a sharp decline afterward, while no MC-LR in kidney was detectable throughout the experiment. Ultrastructural changes in the kidney of crucian carp progressed with increasing accumulated MCs and exposure times within 48 h post-injection, whereas renal ultrastructural recovery of crucian carp in the 50 mu g MC-LReq. kg(-1) dose group was evident at 168 h post-injection. Our ultrastructural observation suggests that the membranous structure is the main action site of MCs in the kidney, among which mitochondria damage in the tubules is clearly an early, and presumably a critically important effect of MCs. The increases in blood urea nitrogen (BUN) and creatinine (CR) in both dose groups further revealed severe impairment occurred in the kidney of crucian carp. (C) 2013 Elsevier Ltd. All rights reserved.