DETERMINATION OF TOTAL AND DIRECT BILIRUBINS IN SERUM WITH SODIUM DODECYL-SULFATE AS AN ACCELERATOR
DETERMINATION OF TOTAL AND DIRECT BILIRUBINS IN SERUM WITH SODIUM DODECYL-SULFATE AS AN ACCELERATOR
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DOI：
10.1016/0009-8981(78)90162-6
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发表时间：
1978-01-01
影响因子：
5
通讯作者：
KAWASHIMA, H
中科院分区：
文献类型：
--
作者：
WATANABE, F;TSUBOTA, N;KAWASHIMA, H
描述了一种使用十二烷基硫酸钠 (SDS) 测定血清中总胆红素和结合胆红素的新方法。在 SDS 存在下，胆红素与重氮化磺胺酸的反应顺利进行，并在室温下 15 分钟内完成，得到在 565 nm 处具有最大吸光度的澄清溶液，该吸光度在 200 mg/l 胆红素浓度水平下呈线性。该方法的优点是反应混合物澄清，结果不受溶血、血脂和浑浊血清的影响。该方法为血清中总胆红素和结合胆红素的测量提供了新的通用应用。
A new method for the determination of total and conjugated bilirubins in serum with use of sodium dodecyl sulfate (SDS) is described. In the presence of SDS, the reaction of bilirubin with diazotized sulfanilic acid proceeded smoothly and was completed within 15 min at room temperature, giving a clear solution having a maximal absorbance at 565 nm which was linear up to a level of 200 mg/l bilirubin concentration. The advantages of this method are that a clear reaction mixture is obtained and the result is not affected by hemolysis, lipemic and turbid sera. The proposed method offers a new general application for the measurement of total and conjugated bilirubins in serum.