Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors
Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors
复制标题
DOI：
10.1073/pnas.93.10.4974
复制
发表时间：
1996-05-14
影响因子：
11.1
通讯作者：
Vaux, DL
中科院分区：
文献类型：
--
作者：
Uren, AG;Pakusch, M;Vaux, DL
杆状病毒凋亡抑制剂（IAP）在昆虫细胞中起防止细胞死亡的作用。本文描述了IAP的三种哺乳动物同源物MIHA、MIHB和MIHC，以及果蝇IAP同源物DIHA。每个蛋白具有三个杆状病毒IAP重复序列和一个N-末端环指基序。MIHA和MIHB也能抑制白细胞介素1 β转化酶（ICE）介导的细胞凋亡，而这种作用可被拟杉毒蛾核型多角体病毒IAP（OpIAP）和复合体病毒crmA所抑制。由于MIHB和MIHC能够在酵母双杂交试验中与肿瘤坏死因子受体相关因子TRAF 1和TRAF 2结合，这些结果表明IAP蛋白抑制细胞凋亡可能是通过调节ICE样蛋白酶激活所需的信号来实现的。
Baculovirus inhibitors of apoptosis (IAPs) act in insect cells to prevent cell death, Here we describe three mammalian homologs of IAP, MIHA, MIHB, and MIHC, and a Drosophila IAP homolog, DIHA. Each protein bears three baculovirus IAP repeats and an N-terminal ring finger motif. Apoptosis mediated by interleukin 1 beta converting enzyme (ICE), which can be inhibited by Orgyia pseudotsugata nuclear polyhedrosis virus IAP (OpIAP) and compos virus crmA, was also inhibited by MIHA and MIHB. As MIHB and MIHC were able to bind to the tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 in yeast two-hybrid assays, these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE-like proteases.