Asymmetric wall ingrowth deposition in Arabidopsis phloem parenchyma transfer cells is tightly associated with sieve elements.
Asymmetric wall ingrowth deposition in Arabidopsis phloem parenchyma transfer cells is tightly associated with sieve elements.
复制标题
拟南芥韧皮部薄壁组织转移细胞的不对称壁向内生长沉积与筛元素紧密相关。
DOI：
10.1093/jxb/erac234
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发表时间：
2022-09-12
影响因子：
6.9
通讯作者：
中科院分区：
文献类型：
--
作者：
壁向内生长沉积在韧皮部薄壁组织转移细胞的拟南芥叶小脉开始附近的筛元素，而不是同伴细胞，并扩大优先覆盖这个接口。在拟南芥中，韧皮部薄壁组织（PP）传递细胞（TC）的壁向内生长的极化沉积发生在筛元/伴细胞（SE/CC）复合体的细胞附近。然而，这些不同的细胞类型之间的空间关系在小静脉，韧皮部加载发生，知之甚少。将PP TC的发育和壁向内生长定位与Col-0和分别鉴定CC和PP细胞的转基因株系AtSUC 2：：AtSTP 9-GFP（绿色荧光蛋白）和AtSWEET 11：：AtSWEET 11-GFP中的其他韧皮部细胞的那些进行比较。小脉中PP TC的发育，以壁向内生长的沉积为标志，在叶中向基瓣方向进行。然而，并不是所有的PP细胞壁向内生长，更高水平的沉积发生在远轴-与近轴定位的PP TC相比。此外，壁内生长的沉积只发生在与SE相邻的PP细胞中，并优先覆盖PP TC/SE界面，而不是PP TC/CC界面。总的来说，这些结果表明SE和PP TC的壁向内生长沉积之间的紧密联系，并建议存在两个亚型的PP细胞在叶小静脉。与PP细胞相比，PP TC显示出更丰富的AtSWEET 11-GFP积累，表明PP和PP TC之间的韧皮部负载的功能差异。
Wall ingrowth deposition in phloem parenchyma transfer cells of Arabidopsis leaf minor veins is initiated adjacent to sieve elements, rather than companion cells, and expands to preferentially cover this interface. In Arabidopsis, polarized deposition of wall ingrowths in phloem parenchyma (PP) transfer cells (TCs) occurs adjacent to cells of the sieve element/companion cell (SE/CC) complex. However, the spatial relationships between these different cell types in minor veins, where phloem loading occurs, are poorly understood. PP TC development and wall ingrowth localization were compared with those of other phloem cells in leaves of Col-0 and the transgenic lines AtSUC2::AtSTP9-GFP (green fluorescent protein) and AtSWEET11::AtSWEET11-GFP that identify CCs and PP cells, respectively. The development of PP TCs in minor veins, indicated by deposition of wall ingrowths, proceeded basipetally in leaves. However, not all PP cells develop wall ingrowths, and higher levels of deposition occur in abaxial- compared with adaxial-positioned PP TCs. Furthermore, the deposition of wall ingrowths was exclusively initiated on and preferentially covered the PP TC/SE interface, rather than the PP TC/CC interface, and only occurred in PP cells that were adjacent to SEs. Collectively, these results demonstrate a tight association between SEs and wall ingrowth deposition in PP TCs and suggest the existence of two subtypes of PP cells in leaf minor veins. Compared with PP cells, PP TCs showed more abundant accumulation of AtSWEET11–GFP, indicating functional differences in phloem loading between PP and PP TCs.
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DOI：
10.1093/plcell/koaa060
发表时间：
2021-05-05
期刊：
The Plant cell
影响因子：
--
作者：
Kim JY;Symeonidi E;Pang TY;Denyer T;Weidauer D;Bezrutczyk M;Miras M;Zöllner N;Hartwig T;Wudick MM;Lercher M;Chen LQ;Timmermans MCP;Frommer WB
通讯作者：
Frommer WB
DOI：
10.1242/dev.127613
发表时间：
2015-12-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kurihara D;Mizuta Y;Sato Y;Higashiyama T
通讯作者：
Higashiyama T
影响因子：
5.6
作者：
Sakurai G;Miklavcic SJ
通讯作者：
Miklavcic SJ
影响因子：
4.3
作者：
McCubbin, Tyler J.;Braun, David M.
通讯作者：
Braun, David M.
影响因子：
7.2
作者：
Stadler, R;Wright, KM;Sauer, N
通讯作者：
Sauer, N