Porphyromonas gingivalis survives within KB cells and modulates inflammatory response
Porphyromonas gingivalis survives within KB cells and modulates inflammatory response
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DOI：
10.1111/j.1399-302x.2006.00282.x
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发表时间：
2006-08-01
影响因子：
--
通讯作者：
Pfister, W.
中科院分区：
文献类型：
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作者：
Eick, S.;Reissmann, A.;Pfister, W.
背景/目标：本研究的目的是探讨牙龈卟啉单胞菌的细胞内生存作为维持牙周炎的一种可能机制。方法：牙龈卟啉单胞菌菌株，菌株ATCC 33277和7个临床分离株，与KB细胞共培养。在感染后3天内测定细胞内细菌的数量。此外，还测量了每孔KB细胞的数量、细胞因子白细胞介素-1 β（IL-1 β）、IL-6、IL-8和肿瘤坏死因子-α（TNF-α）的浓度以及精氨酸特异性酰胺分解活性。结果：所有的牙龈卟啉单胞菌均能在KB细胞中存活，并能在KB细胞中表达IL-1 β、IL-6、IL-8、TNF-α和rgpA。与第3天集落形成单位的降低值相反，与第0天相比，观察到相等和更高水平的16 S rRNA。在感染过程中，精氨酸特异性酰胺分解活性在所有样品中下降。牙龈卟啉单胞菌感染KB细胞后，rgpA mRNA无表达。在用牙龈卟啉单胞菌菌株感染后2天，在所有样品中可检测到IL-8。主成分分析强调了感染后1小时的精氨酸特异性酰胺分解活性与释放的IL-8和IL-8的mRNA表达之间的相关性。结论：牙龈卟啉单胞菌在上皮细胞内存活，可能处于不可培养阶段。侵入细胞调节牙龈卟啉单胞菌的毒力特性以及细胞的炎症反应。
Background/aims: The purpose of the study was to investigate the intracellular survival of Porphyromonas gingivalis as a possible mechanism for maintaining periodontitis.Methods: P. gingivalis strains, the strain ATCC 33277 and seven clinical isolates, were co-cultured with KB cells. The number of intracellular bacteria was determined up to 3 days after infection. In addition, the numbers of KB cells per well, the concentrations of the cytokines interleukin-1 beta (IL-1 beta), IL-6, IL-8 and tumour necrosis factor-alpha (TNF-alpha) and the arginine-specific amidolytic activity were measured. The 16S rRNA of P. gingivalis and the mRNA expression of IL-1 beta, IL-6, IL-8, TNF-alpha and rgpA were also determined.Results: All the P. gingivalis strains studied were able to survive within KB cells. In contrast to the reduced values of colony-forming units at day 3, equal and higher levels of 16S rRNA were seen in comparison to day 0. Arginine-specific amidolytic activity declined in all samples during infection. Expression of mRNA for rgpA was not found after infection of KB cells by P. gingivalis strains. IL-8 was detectable in all samples 2 days after infection with P. gingivalis strains. Principal components analysis underlined a correlation between the arginine-specific amidolytic activity 1 h after infection and both the released IL-8 and the mRNA expression of IL-8. Associations were found between the cultivable numbers of intracellular P. gingivalis and the mRNAs of IL-1, IL-6 and TNF-alpha at the day of infection.Conclusion: The results indicate survival of P. gingivalis within epithelial cells, possibly in a non-cultivable stage. Invasion into cells modulates the virulence properties of P. gingivalis as well as the inflammatory response of the cells.