Generation of a FTO gene knockout human embryonic stem cell line using CRISPR/Cas9 editing
Generation of a FTO gene knockout human embryonic stem cell line using CRISPR/Cas9 editing
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使用 CRISPR/Cas9 编辑生成 FTO 基因敲除人类胚胎干细胞系
DOI：
10.1016/j.scr.2021.102362
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发表时间：
2021-04-29
影响因子：
1.2
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Wei, Cong;Luo, Qian;Huang, He
脂肪量与肥胖相关蛋白（FTO）是首个被发现具有N6 - 甲基腺苷（m6A）去甲基化活性的蛋白。据报道，FTO参与了多种生理和病理过程，包括干细胞分化、性别决定、肿瘤发生与进展。为进一步了解FTO在这些过程中的确切作用，我们利用CRISPR/Cas9介导的基因编辑方法构建了FTO基因敲除的人胚胎干细胞（hESC）系。该细胞系保持了正常的核型、多能性以及三胚层分化潜能，可作为研究FTO蛋白在hESC自我更新与分化中功能的模型。
Fat mass and obesity-associated protein (FTO) is the first protein found to have the activity of N6-methyladenosine (m6A) demethylation. It has been reported that FTO was involved in different physiological and pathological processes, including stem cell differentiation, sex determination, tumorigenesis, and progression. To further understand the exact role of FTO in these processes, we generated a FTO knockout human embryonic stem cell (hESC) line by CRISPR/Cas9 mediated gene editing method. This cell line maintained normal karyotype, pluripotency, and trilineage differentiation potential, which are considered as a model for function studies of the FTO protein in hESC self-renewal and differentiation.