Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis.
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DOI：
10.1371/journal.pone.0035758
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chan J
中科院分区：
文献类型：
--
作者：
Liu YH;Vaghjiani V;Tee JY;To K;Cui P;Oh DY;Manuelpillai U;Toh BH;Chan J
人羊膜上皮细胞（hAEC）具有干细胞样特征和免疫调节特性。在这里，我们证明 hAEC 在体外显着抑制脾细胞增殖，并有效减弱多发性硬化症 (MS) 小鼠模型。 hAEC 治疗显着减少中枢神经系统 (CNS) CD3+ T 细胞和 F4/80+ 单核细胞/巨噬细胞浸润和脱髓鞘。除了已知的前列腺素 E2 (PGE2) 分泌外，我们还报告了 hAEC 利用转化生长因子-β (TGF-β) 进行免疫抑制的新发现。在脾细胞增殖测定中中和 TGF-β 或 PGE2 可显着减少 hAEC 诱导的抑制。 hAEC 处理小鼠的脾细胞显示 Th2 细胞因子转变，且 IL-5 产量显着升高。虽然可以在肺中检测到转移的 CFSE 标记的 hAEC，但在中枢神经系统或淋巴器官中没有检测到。这是第一份记录 hAEC 在 MS 样模型中的治疗效果的报告，表明 hAEC 可能具有用于治疗 MS 的潜力。
Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3+ T cell and F4/80+ monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC treatment. Besides the known secretion of prostaglandin E2 (PGE2), we report the novel finding that hAEC utilize transforming growth factor-β (TGF-β) for immunosuppression. Neutralization of TGF-β or PGE2 in splenocyte proliferation assays significantly reduced hAEC-induced suppression. Splenocytes from hAEC-treated mice showed a Th2 cytokine shift with significantly elevated IL-5 production. While transferred CFSE-labeled hAEC could be detected in the lung, none were identified in the CNS or in lymphoid organs. This is the first report documenting the therapeutic effect of hAEC in a MS-like model and suggest that hAEC may have potential for use as therapy for MS.
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DOI：
10.4049/jimmunol.1003603
发表时间：
2011-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Li H;Nourbakhsh B;Safavi F;Li K;Xu H;Cullimore M;Zhou F;Zhang G;Rostami A
通讯作者：
Rostami A
影响因子：
--
作者：
Miki, Toshio;Marongiu, Fabio;Strom, Stephen C
通讯作者：
Strom, Stephen C
影响因子：
4.4
作者：
RACKE, MK;CANNELLA, B;MCFARLIN, DE
通讯作者：
MCFARLIN, DE
影响因子：
3.5
作者：
Chen, Xiang T.;Chan, Siow T.;Chan, James
通讯作者：
Chan, James
影响因子：
5.2
作者：
Roddy, Gavin W.;Oh, Joo Youn;Prockop, Darwin J.
通讯作者：
Prockop, Darwin J.