Concentrations of urinary neopterin, but not suPAR, positively correlate with age in rhesus macaques
Concentrations of urinary neopterin, but not suPAR, positively correlate with age in rhesus macaques
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DOI：
10.3389/fevo.2022.1007052
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
Eve B. Cooper;Marina M. Watowich;Nina Beeby;C. Whalen;Michael J. Montague;L. Brent;N. Snyder‐Mackler;J. Higham
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文献类型：
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作者：
Eve B. Cooper;Marina M. Watowich;Nina Beeby;C. Whalen;Michael J. Montague;L. Brent;N. Snyder‐Mackler;J. Higham
确定可以非侵入性测量的免疫系统功能中与年龄相关的变化的生物标志物是在自由放养和野生动物种群中进行免疫衰老和炎症研究的重要一步。在本研究中，我们的目的是调查两种尿中的化合物，新蝶呤和suPAR，作为生物标志物的年龄相关的变化，在自由放养的恒河猴（猕猴）人口的免疫激活和炎症的适用性。我们还研究了血液样本中基因转录的年龄相关变化，以了解驱动尿新蝶呤或suPAR年龄相关变化的潜在近似机制。新蝶呤与年龄呈显著正相关，且具有中等的个体内重复性，表明其可作为年龄相关变化的生物标志物。尿中新蝶呤的年龄相关性变化显然不是由新蝶呤的主要信号因子IFN-γ的年龄相关性增加驱动的，而是由CD 14+和CD 14 −单核细胞的年龄相关性增加驱动的。suPAR与年龄无关，并且在个体内具有低重复性，表明它可能更适合于测量急性炎症而不是慢性年龄相关的炎症增加（即，“发炎”）。新蝶呤和suPAR的相关性为25%，这表明它们可能经常发出不同的信号，如果解开，可以提供串联测量时免疫系统功能和炎症的细微差别。
Identifying biomarkers of age-related changes in immune system functioning that can be measured non-invasively is a significant step in progressing research on immunosenescence and inflammaging in free-ranging and wild animal populations. In the present study, we aimed to investigate the suitability of two urinary compounds, neopterin and suPAR, as biomarkers of age-related changes in immune activation and inflammation in a free-ranging rhesus macaque (Macaca mulatta) population. We also investigated age-associated variation in gene transcription from blood samples to understand the underlying proximate mechanisms that drive age-related changes in urinary neopterin or suPAR. Neopterin was significantly positively correlated with age, and had a moderate within-individual repeatability, indicating it is applicable as a biomarker of age-related changes. The age-related changes in urinary neopterin are not apparently driven by an age-related increase in the primary signaler of neopterin, IFN-y, but may be driven instead by an age-related increase in both CD14+ and CD14− monocytes. suPAR was not correlated with age, and had low repeatability within-individuals, indicating that it is likely better suited to measure acute inflammation rather than chronic age-related increases in inflammation (i.e., “inflammaging”). Neopterin and suPAR had a correlation of 25%, indicating that they likely often signal different processes, which if disentangled could provide a nuanced picture of immune-system function and inflammation when measured in tandem.