p160(ROCK), a Rho-associated coiled-coil forming protein kinase, works downstream of Rho and induces focal adhesions
p160(ROCK), a Rho-associated coiled-coil forming protein kinase, works downstream of Rho and induces focal adhesions
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DOI：
10.1016/s0014-5793(97)00107-5
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发表时间：
1997-03-10
期刊：
影响因子：
3.5
通讯作者：
Narumiya, S
中科院分区：
文献类型：
--
作者：
Ishizaki, T;Naito, M;Narumiya, S
p160（ROCK）是一种丝氨酸/苏氨酸蛋白激酶，其选择性地与GTP-Rho结合并被这种结合激活。为了鉴定其功能，我们用p160（ROCK）的野生型和突变体转染HeLa细胞并检查转染细胞的形态学。用含有激酶结构域和卷曲螺旋形成区的野生型和突变体转染诱导粘着斑和应力纤维，而激酶缺陷突变体或仅含激酶结构域的突变体没有观察到诱导作用。此外，Rho诱导的粘着斑和应力纤维的形成被激酶和Rho结合活性缺陷的突变体的共表达所抑制，然而，Rho仍然诱导这些细胞中F-肌动蛋白含量的增加。这些结果表明，p160（ROCK）在Rho下游起作用，诱导粘着斑的形成，Rho诱导的肌动蛋白聚合是由其他效应物介导的。(C)1997年欧洲生物化学学会联合会。
p160(ROCK) is a serine/threonine protein kinase that binds selectively to GTP-Rho and is activated by this binding, To identify its function, we transfected HeLa cells with wild type and mutants of p160(ROCK) and examined morphology of the transfected cells, Transfection with wild type and mutants containing the kinase domain and the coiled-coil forming region induced focal adhesions and stress fibers, while no induction was observed with a kinase-defective mutant or a mutant containing only the kinase domain, Furthermore, Rho-induced formation of focal adhesions and stress fibers was inhibited by co-expression of a mutant defective in both kinase and Rho-binding activities, Rho, however, still induced an increase in F-actin content in these cells. These results suggest that p160(ROCK) works downstream of Rho to induce formation of focal adhesions and that Rho-induced actin polymerization is mediated by other effector(s). (C) 1997 Federation of European Biochemical Societies.