A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry
A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry
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DOI：
10.1016/j.chroma.2009.12.017
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发表时间：
2010-01-29
影响因子：
4.1
通讯作者：
Wang, Y. T.
中科院分区：
文献类型：
--
作者：
Du, G.;Zhao, H. Y.;Wang, Y. T.
建立了微波辅助萃取-超高效液相色谱-二极管阵列检测-飞行时间质谱（UHPLC-DAD-TOF-MS）同时测定葛根中14种酚类化合物的方法。Ohwi和粉葛Pueraria thomsonii Benth.采用中心组合设计法对微波辅助萃取的操作条件进行了优化。结果表明，以65%乙醇为提取溶剂，提取体积为17 mL，提取温度为100 ℃，提取时间为2 min。将Zorbax SB C-18（50 mm x 4.6 mm I.D.，1.8μ m）和梯度洗脱。通过与对照品的保留时间、紫外光谱和飞行时间质谱数据的比较，成功地鉴定了样品中14种化合物的色谱峰。在检测范围内，各标准曲线线性关系良好（r > 0.9997）。日内和日间变化分别小于1.77%和2.88%。该方法已成功地应用于10个样品中所考察的化合物的测定。结果表明，MAE和UHPLC-DAD-TOF-MS联用技术可为板蓝根药材的质量控制提供一种快速的方法。由爱思唯尔公司出版
A microwave-assisted extraction (MAE) and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry (UHPLC-DAD-TOF-MS) method was developed for simultaneous determination of 14 phenolic compounds in the root of Pueraria lobata (Wild.) Ohwi and Pueraria thomsonii Benth. Operational conditions of MAE were optimized by central composite design (CCD). The optimized result was 65% ethanol as extraction solvent, 17 mL of extraction volume, 100 degrees C of extraction temperature and 2 min of hold time. A Zorbax SB C-18 (50 mm x 4.6 mm I.D., 1.8 mu m) and gradient elution were used during the analysis. The chromatographic peaks of 14 investigated compounds in samples were successfully identified by comparing their retention time, UV spectra and TOF mass data with the reference substances. All calibration curves showed good linearity (r > 0.9997) within the test ranges. The infra-day and inter-day variations were less than 1.77% and 2.88%, respectively. The developed method was successfully applied to determine the investigated compounds in 10 samples of Radix Puerariae Lobatae and Radix Puerariae Thomsonii, respectively. The result indicated that MAE and UHPLC-DAD-TOF-MS system might provide a rapid method for the quality control of Radix Puerariae. Published by Elsevier B.V.