S1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryo-passaged, attenuated derivatives
S1 gene sequence heterogeneity of a pathogenic infectious bronchitis virus strain and its embryo-passaged, attenuated derivatives
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DOI：
10.1080/03079450701338730
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发表时间：
2007-06-01
期刊：
影响因子：
2.8
通讯作者：
Rong, Jungong
中科院分区：
文献类型：
--
作者：
Liu, Shengwang;Han, Zongxi;Rong, Jungong
鸡传染性支气管炎病毒CK/CH/LDL/971经鸡胚连续传代致弱。通过对接种至15日龄无特定病原体鸡的临床反应确定病毒或第115代减毒。接种-攻毒试验表明，减毒的第115代病毒可通过临床应答提供对同源致病性病毒第5代的保护。根据S1基因的序列分析和比较，致病性和减毒性CK/CH/LDL/97-1病毒均为至少包含两个亚群的群体。此外，从S I基因的氨基酸和核苷酸序列的分析，我们推测，次要和显性亚群之间的重组加上突变的积累;在致病性第5代的S1区域可能导致胚胎传代，减毒第115代的形成。
Infectious bronchitis virus CK/CH/LDL/971 was attenuated by serial passage in chicken embryos. Virus or passage 115 was attenuated as determined by clinical response to inoculation to 15-day-old specific pathogen free chickens. The vaccination-challenge test showed that the attenuated passage 115 virus could afford protection against the homologous pathogenic virus, passage 5, by the clinical response. Based on the seqaence analysis and comparison of the S1 gene, both the pathogenic and attenuated CK/CH/LDL/97-1 viruses were populations that each included at least two subpopulations. Also, from analysis of the amino acid and nucleotide sequences of S I gene, we speculate that recombination between the minor and dominant subpopulations plus accumulation of mutations; in the S1 region of pathogenic passage 5 might lead to the formation of the embryo-passaged, attenuated passage 115.