Notch signalling and the synchronization of the somite segmentation clock
Notch signalling and the synchronization of the somite segmentation clock
复制标题
DOI：
10.1038/35044091
复制
发表时间：
2000-11-23
期刊：
影响因子：
64.8
通讯作者：
Lewis, J
中科院分区：
文献类型：
--
作者：
Jiang, YJ;Aerne, BL;Lewis, J
在Notch细胞-细胞通讯途径突变的脊椎动物中，分割失败：划分体节的边界，胚胎体轴的片段，不存在或不规则（1-8）。这种表型促使了许多研究，但Notch信号在体节发生中的作用仍然是神秘的（1，9 -12）。体节模式被认为是由“时钟和波前”机制控制的（13）：一个生化振荡器（分割时钟）在减数分裂前中胚层的细胞中起作用，未成熟的组织从其顺序产生体节，成熟的波前扫过这个组织，阻止振荡并启动体节分化（14，15）。在其周期的不同阶段停滞的细胞表达不同的基因，定义体节的空间周期模式并控制分割的物理过程（1，16 -19）。人们可能会认为，缺口信号对振荡或组织随后的事件（这些事件产生了体节边界）必不可少。在这里，我们分析了一组斑马鱼突变体，并得出一个不同的解释：Notch信号在体节分割的基本功能是保持相邻的preomitic中胚层细胞的振荡同步。
In vertebrates with mutations in the Notch cell-cell communication pathway, segmentation fails: the boundaries demarcating somites, the segments of the embryonic body axis, are absent or irregular(1-8). This phenotype has prompted many investigations, but the role of Notch signalling in somitogenesis remains mysterious(1,9-12). Somite patterning is thought to be governed by a "clock-and-wavefront" mechanism(13) : a biochemical oscillator (the segmentation clock) operates in the cells of the presomitic mesoderm, the immature tissue from which the somites are sequentially produced, and a wavefront of maturation sweeps back through this tissue, arresting oscillation and initiating somite differentiation(14,15). Cells arrested in different phases of their cycle express different genes, defining the spatially periodic pattern of somites and controlling the physical process of segmentation(1,16-19). Notch signalling, one might think, must be necessary for oscillation, or to organize subsequent events that create the somite boundaries. Here we analyse a set of zebrafish mutants and arrive at a different interpretation: the essential function of Notch signalling in somite segmentation is to keep the oscillations of neighbouring presomitic mesoderm cells synchronized.