AQP5 is differentially regulated in astrocytes during metabolic and traumatic injuries
AQP5 is differentially regulated in astrocytes during metabolic and traumatic injuries
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AQP5 在代谢和创伤性损伤期间星形胶质细胞中受到差异性调节
DOI：
10.1002/glia.22555
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发表时间：
2013-10-01
期刊：
影响因子：
6.2
通讯作者：
Yu, Albert Cheung Hoi
中科院分区：
文献类型：
--
作者：
Chai, Rui Chao;Jiang, Jiao Hua;Yu, Albert Cheung Hoi
水运动在大脑的生理和病理状态中起着至关重要的作用。星形胶质细胞负责调节这种水运动，并且是病理条件下脑水肿的主要贡献者。星形胶质细胞中的水通道蛋白（AQP）在脑内水运动的调节中起着关键作用。AQP 1、3、4、5、8和9已被报道存在于大脑中。与AQP 1、4和9相比，AQP 3、5和8的研究较少。在鲜为人知的AQP中，在中枢神经系统外具有多种功能的AQP 5也被指示参与星形胶质细胞的缺氧损伤。在我们的研究中，在星形胶质细胞和神经元的原代培养物中都可以检测到AQP 5表达，并且在1至4周龄的星形胶质细胞原代培养物中证实了星形胶质细胞中的AQP 5表达。AQP 5定位于星形胶质细胞的胞质膜和胞浆中。AQP 5表达在缺血治疗期间下调，在划痕创伤损伤后上调，这也在体内大脑中动脉闭塞模型和刺伤损伤模型中得到证实。在划痕损伤后增加的AQP 5被极化到划痕伤口前缘的星形胶质细胞的迁移过程和细胞质膜，并且AQP 5过表达促进了划痕损伤后星形胶质细胞过程的伸长。总之，这些结果表明，AQP 5可能是一个重要的水通道，在星形胶质细胞的差异表达在各种脑损伤。GLIA 2013;61：1748-1765
Water movement plays vital roles in both physiological and pathological conditions in the brain. Astrocytes are responsible for regulating this water movement and are the major contributors to brain edema in pathological conditions. Aquaporins (AQPs) in astrocytes play critical roles in the regulation of water movement in the brain. AQP1, 3, 4, 5, 8, and 9 have been reported in the brain. Compared with AQP1, 4, and 9, AQP3, 5, and 8 are less studied. Among the lesser known AQPs, AQP5, which has multiple functions identified outside the central nervous system, is also indicated to be involved in hypoxia injury in astrocytes. In our study, AQP5 expression could be detected both in primary cultures of astrocytes and neurons, and AQP5 expression in astrocytes was confirmed in 1‐ to 4‐week old primary cultures of astrocytes. AQP5 was localized on the cytoplasmic membrane and in the cytoplasm of astrocytes. AQP5 expression was downregulated during ischemia treatment and upregulated after scratch‐wound injury, which was also confirmed in a middle cerebral artery occlusion model and a stab‐wound injury model in vivo. The AQP5 increased after scratch injury was polarized to the migrating processes and cytoplasmic membrane of astrocytes in the leading edge of the scratch‐wound, and AQP5 over‐expression facilitated astrocyte process elongation after scratch injury. Taken together, these results indicate that AQP5 might be an important water channel in astrocytes that is differentially expressed during various brain injuries. GLIA 2013;61:1748–1765