Cytotoxic T lymphocyte associated antigen-4 (CTLA-4)+49 A>G gene polymorphism in Egyptian cases with rheumatoid arthritis
Cytotoxic T lymphocyte associated antigen-4 (CTLA-4)+49 A>G gene polymorphism in Egyptian cases with rheumatoid arthritis
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DOI：
10.1016/j.gene.2014.12.046
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发表时间：
2015-03-01
期刊：
影响因子：
3.5
通讯作者：
Salama, Afrah
中科院分区：
文献类型：
--
作者：
Elshazli, Rami;Settin, Ahmad;Salama, Afrah
背景资料：编码细胞毒性T淋巴细胞相关抗原-4（CTL 4 -4）的基因已被报道与几个种族人群中的类风湿关节炎（RA）相关。这项工作的目的是评估这种多态性与RA的易感性，活动和功能障碍在EgyptianSubjects.Subjects和方法：本研究包括112无关RA埃及患者进行了比较，122名健康对照来自同一地点。对于所有受试者，使用PCR-RFLP技术对DNA进行CTLA-4 + 49 A>G（rs 231775）多态性的基因分型。结果：CTIA-4 G等位基因频率在病例组显著高于对照组（37.1%vs.23.4%，OR = 1.93，95%CI = 1.29-2.89，p = 0.002）。CTLA-4 + 49 Gallele携带（AG +GG基因型）频率在RA患者中显著高于对照组（61.6%vs.41.8%，OR = 2.23，95%CI = 132-3.77，p = 0.003）。Logistic回归分析显示，G等位基因（GA +GG基因型）阳性的病例类风湿畸形的频率较低，也较低的DAS 28-CRP评分，但具有较高的视觉模拟评分（VAS），即更多的功能残疾比其他cases.Conclusions：CTL 9 -4 + 49 G等位基因携带与增加的易感性和功能残疾的RA在埃及患者。(C)2014爱思唯尔有限公司版权所有。
Background: The gene encoding cytotoxic T lymphocyte associated antigen-4 (CTL4-4) has been reported to be associated with rheumatoid arthritis (RA) in several ethnic populations. The aim of this work is to assess the association of this polymorphism with the susceptibility, activity and functional disability of RA in Egyptian subjects.Subjects and methods: This study included 112 unrelated RA Egyptian patients who were compared to 122 healthy controls from the same locality. For all subjects, DNA was genotyped for CTLA-4 +49 A>G (rs231775) polymorphism using the PCR-RFLP technique. Antibodies to cyclic citrullinated peptides (anti-CCP) were measured by enzyme-linked immunosorbent assay (ELISA).Results: The frequency of the CTIA-4 G allele was significantly higher among cases compared to controls (37.1% vs. 23.4%, OR = 1.93; 95% CI = 1.29-2.89, p = 0.002). Also, the frequency of CTLA-4 +49 Gallele carriage (AG +GG genotypes) was significantly higher among cases with RA compared to controls (61.6% vs. 41.8%, OR = 2.23,95% Cl = 132-3.77, p = 0.003). Logistic regression analysis showed that cases positive to the G allele (GA +GG genotypes) had less frequency of rheumatoid deformities and also a lower DAS28-CRP score, yet with a higher visual analogue scale (VAS) i.e. more functional disability than other cases.Conclusions: CTL9-4 +49 G allele carriage was associated with increased susceptibility and functional disability of RA in Egyptian patients. (C) 2014 Elsevier B.V. All rights reserved.