Characterization of Salmonella resistance to bile during biofilm formation.
Characterization of Salmonella resistance to bile during biofilm formation.
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DOI：
10.1016/j.jmii.2019.06.003
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发表时间：
2020-08
期刊：
影响因子：
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通讯作者：
M. Tsai;Yi-Hua Liang;Chyi-Liang Chen;C. Chiu
中科院分区：
文献类型：
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作者：
M. Tsai;Yi-Hua Liang;Chyi-Liang Chen;C. Chiu
背景：非伤寒沙门氏菌感染可表现为急性胃肠炎或慢性感染，主要发生在富含胆汁的胆囊。生物膜的形成是沙门氏菌胆汁耐药的一种机制。我们的目的是确定沙门氏菌如何利用胆汁作为信号，并研究PhoP-PhoQ双组分系统和环二氨基磷酸（c-di-GMP）信号之间的相互作用与生物膜形成的相关性。方法采用二维凝胶电泳技术鉴定胆汁中沙门氏菌生物膜形成所需基因。利用实时荧光定量PCR （qRT-PCR）研究了PhoP-PhoQ双组分系统在生物膜形成过程中的作用及其与c-di-GMP网络相关基因的相互作用。结果外膜不完整（△ompA）、鞭毛缺陷（△flgE）或phoP - phoq双组分系统不完整（△phoP）的沙门氏菌突变体在胆汁存在下不能形成完整的生物膜。此外，phoP - phoQ双组分系统相关的沙门氏菌突变体（△phoP、△phoQ、△phoP△phoQ）在胆汁环境中c-二gmp相关基因（csgD、adrA）的表达低于野生型沙门氏菌。结论沙门氏菌在生物膜形成过程中可能通过PhoP-PhoQ双组分系统对胆汁进行感知和应答。此外，PhoP-PhoQ双组分系统可能激活c-di-GMP信号网络的调节因子。
BackgroundNon-typhoidSalmonellainfection may present as acute gastroenteritis or chronic infection, primarily in the bile-rich gallbladder. Biofilm formation is a mechanism of bile resistance inSalmonella. Our aim was to determine howSalmonellautilizes bile as a signal, and to study the relevance of the interaction between the PhoP-PhoQ two-component system and cyclic diguanosine monophosphate (c-di-GMP) signaling to biofilm formation.MethodsTwo-dimensional (2-D) gel electrophoresis was used to identify genes required forSalmonellabiofilm formation in bile. Quantitative real-time PCR (qRT-PCR) was used to clarify the role of the PhoP-PhoQ two-component system and its interaction with genes involved in the c-di-GMP network during biofilm formation.ResultsOur result revealed thatSalmonellamutants with incomplete outer membrane (△ompA), defective flagella (△flgE), or incomplete PhoP-PhoQ two-component system (△phoP), were unable to develop complete biofilms in the presence of bile. Moreover, PhoP-PhoQ two-component system-relatedSalmonellamutants (△phoP, △phoQ, △phoP△phoQ) had lower expression of c-di-GMP related genes (csgD,adrA) than the wild-typeSalmonellastrain had in the bile environment.ConclusionSalmonellamay sense and respond to bile through the PhoP-PhoQ two-component system during biofilm formation. Furthermore, the PhoP-PhoQ two-component system might activate regulators of the c-di-GMP signaling network.