SUB-PICONEWTON FORCE FLUCTUATIONS OF ACTOMYOSIN INVITRO
SUB-PICONEWTON FORCE FLUCTUATIONS OF ACTOMYOSIN INVITRO
复制标题
DOI：
10.1038/352301a0
复制
发表时间：
1991-07-25
期刊：
影响因子：
64.8
通讯作者：
YANAGIDA, T
中科院分区：
文献类型：
--
作者：
ISHIJIMA, A;DOI, T;YANAGIDA, T
一种新的系统已经开发出来，用于测量由少量（< 5-150）的肌球蛋白分子与单个肌动蛋白丝在体外相互作用所产生的力。该技术可以解决小于皮牛顿的力，并具有亚毫秒范围内的时间分辨率。因此，它可以检测由单个分子相互作用引起的力的波动。从这些力的波动分析，肌动球蛋白的酶ATP酶活性和由此产生的机械脉冲之间的耦合可以阐明。
A new system has been developed for measuring the forces produced by a small number (< 5-150) of myosin molecules interacting with a single actin filament in vitro. The technique can resolve forces of less than a piconewton and has a time resolution in the submillisecond range. It can thus detect fluctuations of force caused by individual molecular interactions. From analysis of these force fluctuations, the coupling between the enzymatic ATPase activity of actomyosin and the resulting mechanical impulses can be elucidated.