PRODUCTION OF VITAMIN-B12 ANALOGS IN PATIENTS WITH SMALL BOWEL BACTERIAL OVERGROWTH
PRODUCTION OF VITAMIN-B12 ANALOGS IN PATIENTS WITH SMALL BOWEL BACTERIAL OVERGROWTH
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DOI：
10.7326/0003-4819-87-5-546
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发表时间：
1977-01-01
影响因子：
39.2
通讯作者：
WAGLE, A
中科院分区：
文献类型：
--
作者：
BRANDT, LJ;BERNSTEIN, LH;WAGLE, A
研究了4例细菌过度生长患者肠道内容物中维生素B12类似物（钴酰胺）的存在情况以及57 Co-B12（维生素B12氰钴胺，[57 Co]-CN-Cbl）向钴酰胺的细菌转化。口服内因子结合的（57 Co）-CN-Cbl。抽取空肠内容物24 h，进行需氧和厌氧培养。通过电泳和色谱分离CN-Cbl和钴酰胺，并通过生物自显影鉴定。一式两份色谱图中钴酰胺区域的放射性表明，（57 Co）-CN-Cbl在细菌作用下转化为钴酰胺。在4例患者中的3例中，在肠内容物中发现了钴酰胺（[Ade] CNCba、[2-Me Ade] CNCba、[CN]2Cbi和因子E），在3例患者中的2例中，钴酰胺占CN-Cbl给药量的25%以上。在细菌过度生长状态下，人体会重新产生钴酰胺，并通过摄入与内在因子结合的氯化萘-Cbl产生钴酰胺，导致宿主大量损失维生素B12。
The presence of vitamin B12 analogues (cobamides) and bacterial conversion of 57Co-B12 (vitamin B12 cyanocobalamin, [57Co]-CN-Cbl) into cobamides in intestinal contents of 4 patients with bacterial overgrowth were investigated. The (57Co)-CN-Cbl bound to intrinsic factor was given orally. Jejunal contents were aspirated for 24 h and cultured aerobically and anaerobically. The CN-Cbl and cobamides were separated by electrophoresis and chromatography and identified by bioautography. Radioactivity of cobamide zones from duplicate chromatograms showed bacterial conversion of (57Co)-CN-Cbl into cobamides. Cobamides ([Ade]CNCba, [2-Me Ade] CNCba, [CN]2Cbi and factor E) were found in intestinal contents in 3 of 4 patients, and in 2 of 3 patients cobamides represented more than 25% of administered CN-Cbl. Bacterial production of cobamides, de novo and from ingested CN-Cbl bound to intrinsic factor, occurs in humans with bacterial overgrowth states and results in a significant loss of vitamin B12 to the host.