Opportunities for mesoporous nanocrystalline SnO2 electrodes in kinetic and catalytic analyses of redox proteins
Opportunities for mesoporous nanocrystalline SnO2 electrodes in kinetic and catalytic analyses of redox proteins
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DOI：
10.1042/bst0370368
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发表时间：
2009-04-01
影响因子：
3.9
通讯作者：
Butt, Julea N.
中科院分区：
文献类型：
--
作者：
Kemp, Gemma L.;Marritt, Sophie J.;Butt, Julea N.
PFV（蛋白质膜伏安法）可以对氧化还原和偶联化学事件进行动力学分析。然而，伏安图报告了通过电流流动的电子转移，因此需要同步光谱才能对所涉及的物种进行化学了解。介孔纳米晶 SnO2 电极通过其高表面积和可见波长下的光学透明度为此类“光谱电化学”分析提供了机会。在这里，我们通过对大肠杆菌 NrfA（一种具有亚硝酸盐和一氧化氮还原酶活性的五血红素细胞色素）的研究，说明了使用此类电极可以提供的动力学和机制见解。此外，我们证明，通过 MCD（磁圆二色性）光谱表征电催化活性蛋白质膜的能力是一项进步，最终将有助于我们解决许多氧化还原酶中的催化中间体的努力。
PFV (protein film voltammetry) allows kinetic analysis of redox and coupled-chemical events. However, the voltammograms report on the electron transfer through a flow of electrical current such that simultaneous spectroscopy is required for chemical insights into the species involved. Mesoporous nanocrystalline SnO2 electrodes provide opportunities for such 'spectroelectrochemical' analyses through their high surface area and optical transparency at visible wavelengths. Here, we illustrate kinetic and mechanistic insights that may be afforded by working with such electrodes through studies of Escherichia coli NrfA, a pentahaem cytochrome with nitrite and nitric oxide reductase activities. In addition, we demonstrate that the ability to characterize electrocatalytically active protein films by MCD (magnetic circular dichroism) spectroscopy is an advance that should ultimately assist our efforts to resolve catalytic intermediates in many redox enzymes.