Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila.
Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila.
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DOI：
10.17912/micropub.biology.000387
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发表时间：
2021
影响因子：
--
通讯作者：
Nakato H
中科院分区：
文献类型：
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作者：
Takemura M;Lu YS;Nakato E;Nakato H
Unpaired 1（Upd 1）是果蝇Janus激酶/信号转导和转录激活因子（JAK/STAT）信号通路的配体。在这项研究中，使用CRISPR/Cas9技术，我们产生了一种转基因果蝇品系，其中血凝素（HA）表位标签序列插入到upd 1基因的内源性基因座中。抗HA抗体染色证实，表位标记的Upd 1：：HA在各种组织中的分布与先前研究揭示的upd 1表达模式一致。这种转基因果蝇品系将有助于研究Upd 1的表达、定位和关联伙伴，从而有助于理解JAK/STAT通路的激活是如何调节的。
Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in Drosophila. In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the endogenous locus of the upd1 gene. Anti-HA antibody staining confirms that the distribution of the epitope-tagged Upd1::HA in various tissues is consistent with upd1 expression patterns revealed by previous studies. This transgenic fly strain will be useful in studying the expression, localization, and association partners of Upd1, and thus will contribute to understanding how activation of the JAK/STAT pathway is regulated.