XIST unmethylated DNA fragments in male-derived plasma as a tumour marker for testicular cancer
XIST unmethylated DNA fragments in male-derived plasma as a tumour marker for testicular cancer
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DOI：
10.1016/s0140-6736(03)15170-7
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发表时间：
2004-01-03
期刊：
影响因子：
168.9
通讯作者：
Okada, Y
中科院分区：
文献类型：
--
作者：
Kawakami, T;Okamoto, K;Okada, Y
睾丸生殖细胞肿瘤（TGCT）是20-40岁男性中最常见的恶性疾病。我们根据肿瘤的非甲基化DNA谱开发了TGCT的DNA肿瘤标记物。XIST基因的5'末端在TGCT中主要是低甲基化的，而与XIST表达无关。然而，男性体细胞通过CpG位点显示完全甲基化，包括最小启动子和XIST保守重复序列。在男性血浆中鉴定XIST未甲基化片段可能是TGCT的诊断。
Testicular germ-cell tumours (TGCTs) are the most common malignant diseases among men aged 20-40 years. We developed a DNA tumour marker for TGCTs based on the unmethylated DNA profile of a neoplasm. The 5' end of the XIST gene is mainly hypomethylated in TGCTs irrespective of XIST expression. Male somatic cells, however, show complete methylation through the CpG sites, including the minimum promoter and XIST-conserved repeats. Identification of a XIST unmethylated fragment in male plasma might be diagnostic for TGCTs.