Distribution of selenium and glutathione peroxidase in blood fractions from humans, rhesus and squirrel monkeys, rats and sheep.
Distribution of selenium and glutathione peroxidase in blood fractions from humans, rhesus and squirrel monkeys, rats and sheep.
复制标题
人类、恒河猴、松鼠猴、大鼠和绵羊血液成分中硒和谷胱甘肽过氧化物酶的分布。
DOI：
10.1093/jn/113.11.2138
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
P. Whanger
中科院分区：
文献类型：
--
作者：
M. A. Beilstein;P. Whanger
用Sephadex G-150凝胶过滤法研究了人、猕猴、松鼠、绵羊和大鼠血中Se和GSH-Px的分布。用GSH-Px共层析测定了绵羊、松鼠、猴和大鼠血浆和红细胞中的大部分硒。相比之下，人类和恒河猴血浆中的硒与两种非GSH-Px蛋白共层析，以及几乎所有红细胞中的硒与血红蛋白峰共层析。恒河猴和人红细胞中有大量的GSH-Px活性也与血红蛋白组分共层析，但很少的GSH-Px与来自松鼠猴、绵羊和大鼠血的血红蛋白洗脱。结果表明，GSH-Px活性可能不能很好地衡量高等灵长类动物的硒状况。
The distribution of selenium and glutathione peroxidase (GSH-Px) was investigated in blood fractions from humans, rhesus and squirrel monkeys, sheep and rats by gel filtration (Sephadex G-150). The majority of selenium in plasma and erythrocytes from sheep, squirrel monkeys and rats cochromatographed with GSH-Px. In contrast, selenium in plasma from humans and rhesus monkeys cochromatographed with two non-GSH-Px proteins and nearly all of the selenium in erythrocytes cochromatographed with the hemoglobin peak. A significant amount of GSH-Px activity in rhesus monkey and human erythrocytes also cochromatographed with the hemoglobin fraction, but very little GSH-Px coeluted with hemoglobin from squirrel monkeys, ovine and rat blood. The results suggest that GSH-Px activity may not be a good measure of selenium status in higher primates.