Examination of Antioxidative System's Responses in the Different Phases of Drought Stress and During Recovery in Desert Plant Reaumuria soongorica (Pall.) Maxim
Examination of Antioxidative System's Responses in the Different Phases of Drought Stress and During Recovery in Desert Plant Reaumuria soongorica (Pall.) Maxim
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DOI：
10.1007/s12374-009-9053-7
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发表时间：
2009-10-01
影响因子：
2.9
通讯作者：
Wang, Gang
中科院分区：
文献类型：
--
作者：
Bai, Juan;Gong, Chun-Mei;Wang, Gang
以红砂（Reaumuria soongorica（Pall.））为试材，研究了红砂干化过程不同阶段超氧化物歧化酶（SOD）、愈创木酚过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）对氧化损伤的保护作用及其活性变化。Maxim.叶子在连续100天期间施加干旱胁迫，并在16、72和100天后复水。随着干旱胁迫的进行，过氧化氢（H2 O2）、丙二醛（MDA）和超氧化物歧化酶（SOD）活性显著升高。POD和CAT活性在干旱胁迫初期显著升高，随着干旱胁迫的继续，POD活性显著降低，复水后POD活性无法恢复。抗坏血酸、还原型谷胱甘肽、APX和GR活性在干旱初期下降，随着水分亏缺程度的增加而显著升高，复水后恢复。这些结果表明：结果表明：（1）在整个干旱胁迫过程中，铁对超氧阴离子的清除作用非常明显;（2）CAT在干旱胁迫早期清除H2 O2，抗坏血酸-谷胱甘肽循环酶在进一步加剧干旱胁迫时清除H2 O2;（3）POD在干旱胁迫下对H2 O2引起的氧化损伤没有保护作用。
The aim of this study was to test the protective roles of superoxide dismutases (SODs), guaiacol peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) against oxidative damage and their activities in different phases of the dry down process in Reaumuria soongorica (Pall.) Maxim. leaves. Drought stress was imposed during 100 consecutive days and rewatering after 16, 72, and 100 days. The concentration of hydrogen peroxide (H2O2), malondialdehyde, and SODs activities were elevated significantly with progressing drought stress. POD and CAT activities increased markedly in the early phase of drought and decreased significantly with further drought stress continuation, and POD activity was unable to recover after rewatering. Ascorbate, reduced glutathione, APX, and GR activities declined in the initial stages of drought process, elevated significantly with further increasing water deficit progression and recovered after rewatering. These results indicate that: (1) iron SODs-removing superoxide anion is very effective during the whole drought stress; (2) CAT scavenges H2O2 in the early phase of drought and enzymes of ascorbate-glutathione cycle scavenge H2O2 in further increasing drought stress; and (3) POD does not contribute to protect against oxidative damage caused by H2O2 under drought stress.