EFFECT OF CALCIUM ON INTRACELLULAR SODIUM + POTASSIUM CONCENTRATIONS IN PLANT + ANIMAL CELLS
EFFECT OF CALCIUM ON INTRACELLULAR SODIUM + POTASSIUM CONCENTRATIONS IN PLANT + ANIMAL CELLS
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DOI：
10.1038/204641a0
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发表时间：
1964-01-01
期刊：
影响因子：
64.8
通讯作者：
ROBBINS, E
中科院分区：
文献类型：
--
作者：
MORRILL, GA;KABACK, HR;ROBBINS, E
测定了小鼠胰腺、R. pipiens（frog）卵母细胞、HeLa细胞、腹水瘤细胞、酵母和E.大肠杆菌在含钙培养基和无钙EDTA培养基中培养后进行了比较。除了E。coli中，细胞内Na含量始终低于细胞外Na含量。所有的细胞系统保持K梯度的存在下，细胞外钙，细胞内：细胞外K的比例范围从6到80。在无钙EDTA中，所有细胞系统都增加了Na，损失了K。这是不阻止镁的存在下，很容易逆转的细胞转移到含钙的培养基。这些结果表明，细胞外Ca ~（2+）离子是维持生命系统中细胞内低Na和高K的必要条件。这一要求的少数例外情况是由于pH值不受控制，目前正在调查中。P. J.刷。
The intracellular Na and K concentrations of mouse pancreas,R. pipiens(frog) oocytes, HeLa cells, ascites tumour cells, yeast andE. coliwere compared after incubation in Ca-containing medium and in Ca-free EDTA medium. With the exception ofE. coli, intracellular Na was always less than extracellular Na. All cell systems maintained a K gradient in the presence of extracellular Ca, the ratio of intracellular: extracellular K ranging from 6 to 80. In Ca-free EDTA, all cell systems gained Na and lost K. This was not prevented by the presence of Mg and was readily reversed by transfer of the cells to Ca-containing medium. These results suggest that extracellular Ca ion is required for the maintenance of low intracellular Na and high intracellular K in living systems. The few reported exceptions to this requirement are attributed to uncontrolled conditions of pH and are being investigated.-P. J. BRUSH.