Seasonal variations of tris (2-chloroethyl) phosphate and cytotoxicity of organic extracts in water samples from Wuhan, China.
Seasonal variations of tris (2-chloroethyl) phosphate and cytotoxicity of organic extracts in water samples from Wuhan, China.
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DOI：
10.1016/j.jes.2018.05.013
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发表时间：
2019-02
影响因子：
6.9
通讯作者：
Youjian Zhang;Wenjuan Zhang;Jian Hou;Xian Wang;Wenhong Lu;Hongyan Zheng;W. Xiong;Junling Liu;Jing Yuan
中科院分区：
文献类型：
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作者：
Youjian Zhang;Wenjuan Zhang;Jian Hou;Xian Wang;Wenhong Lu;Hongyan Zheng;W. Xiong;Junling Liu;Jing Yuan
磷酸三（2-氯乙基）酯（TCEP）是一种典型的磷酸酯类阻燃剂。其潜在的不良健康影响最近引起了人们的关注。研究了中国两个饮用水处理厂（DWTPs）原水、成品水和自来水中TCEP浓度的季节性变化，并评价了水样中有机提取物（OEs）的细胞毒性和细胞凋亡/坏死。采用固相萃取法富集水样中的TCEP和OEs。采用气相色谱-质谱法测定水样中TCEP的浓度。采用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物法和流式细胞术检测水样中OEs对正常人肝细胞株L02的细胞毒性和凋亡/坏死的影响。结果表明，两个水厂原水中OEs的细胞毒性夏、冬两季均强于春、秋两季，两个水厂出厂水和自来水中OEs的细胞毒性夏、秋两季均强于春、冬两季。在所有季节，最大浓度（100 mL水/mL细胞培养物）的OEs在原水样品从两个DWTP诱导晚期凋亡/坏死。水样中TCEP季节性变化的原因以及水样中其他污染物的潜在毒性效应有待进一步研究。
Tris (2-chloroethyl) phosphate (TCEP) is a typical phosphate flame retardant. Its potential adverse health effects have recently aroused concern. We investigated the seasonal variations of TCEP concentrations in the raw, finished and tap water samples from two drinking water treatment plants (DWTPs) in China, and evaluated the cytotoxicity and apoptosis/necrosis of organic extracts (OEs) in water samples. We enriched TCEP and OEs in water samples by solid-phase extraction method. The TCEP concentrations in water samples were determined by gas chromatography–mass spectrometry. Normal human liver cell line L02 was treated with OEs in the water samples, and then the cytotoxicity and apoptosis/necrosis were measured by 3-(4, 5-dimethyithiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and flow cytometry, respectively. The results showed that cytotoxicities of OEs in raw water samples from both DWTPs in summer and winter were stronger than those in spring and autumn, cytotoxicity of OEs in finished and tap water samples from both DWTPs in summer and autumn were stronger than those in spring and winter. In all seasons, the maximal concentrations (100 mL water/mL cell culture) of OEs in the raw water samples from both DWTPs induced late apoptosis/necrosis. The reasons for seasonal variations of TCEP in water samples and potential toxic effects of other pollutants in the water samples need to be further investigated.