Differential localization of carbohydrate epitopes in plant cell walls in the presence and absence of arbuscular mycorrhizal fungi
Differential localization of carbohydrate epitopes in plant cell walls in the presence and absence of arbuscular mycorrhizal fungi
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DOI：
10.1104/pp.111.1.203
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发表时间：
1996-05-01
期刊：
影响因子：
7.4
通讯作者：
Bonfante, P
中科院分区：
文献类型：
--
作者：
Balestrini, R;Hahn, MG;Bonfante, P
针对鼠李糖半乳糖醛酸 I 生成的两种单克隆抗体 (McAb) 具有对末端 α-(1-->2) 连接的岩藻糖基表位 (CCRC-M1) 和阿拉伯糖基化 β-(1,6)-半乳聚糖表位 (CCRC-M7) 的特异性，用于免疫金实验以确定表位在四种植物中的分布。选择葱、玉米、白车轴草和烟草植物作为具有不同壁结构的单子叶植物和双子叶植物的代表。在丛枝菌根真菌存在和不存在的情况下对根组织进行分析。在组织之间和物种之间发现了两个细胞壁表位的差异定位：例如，在韭菜中，CCRC-M1 标记表皮和皮下细胞，而 CCRC-M7 仅标记皮质细胞。三叶草壁均由两种单克隆抗体标记，而玉米和烟草仅由 CCRC-M7 标记。在丛枝菌根真菌存在的情况下，在细胞内菌丝周围发生的典型共生（界面）的质外体隔室中还发现了标记。在界面材料中发现了结合两种单克隆抗体的表位，它们的分布反映了在宿主细胞壁中发现的模式。这些发现表明，真菌-植物共生中界面区的组成反映了宿主细胞壁的组成。
Two monoclonal antibodies (McAbs) generated against rhamnogalacturonan I and characterized as specific for a terminal alpha-(1-->2)-linked fucosyl-containing epitope (CCRC-M1) and for an arabinosylated beta-(1,6)-galactan epitope (CCRC-M7) were used in immunogold experiments to determine the distribution of the epitopes in four plants. Allium porrum, Zea mays, Trifolium repens, and Nicotiana tabacum plants were chosen as representatives of monocots and dicots with different wall structures. Analyses were performed on root tissues in the presence and absence of arbuscular mycorrhizal fungi. A differential localization of the two cell wall epitopes was found between tissues and between species: for example, in leek, CCRC-M1 labeled epidermal and hypodermal cells, whereas CCRC-M7 labeled cortical cells only. Clover walls were labeled by both McAbs, whereas maize and tobacco were only labeled by CCRC-M7. In the presence of the arbuscular mycorrhizal fungi, labeling was additionally found in an apoplastic compartment typical of the symbiosis (the interface) occurring around the intracellular hyphae. Epitopes binding both McAbs were found in the interfacial material, and their distribution mirrored the pattern found in the host cell wall. These findings demonstrate that the composition of the interface zone in a fungus-plant symbiosis reflects the composition of the wall of the host cell.