Analysis of the effects of surface treatments on nickel release from nitinol wires and their impact on candidate gene expression in endothelial cells
Analysis of the effects of surface treatments on nickel release from nitinol wires and their impact on candidate gene expression in endothelial cells
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DOI：
10.1007/s10856-006-0087-9
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发表时间：
2008-03-01
影响因子：
3.7
通讯作者：
Smith, T. J.
中科院分区：
文献类型：
--
作者：
McLucas, E.;Rochev, Y.;Smith, T. J.
镍钛诺在医疗器械行业中有许多应用，但是大量的镍，一种已知的过敏原和致癌物仍然是一个严重的问题。研究已经表明，镍离子诱导一系列基因的差异表达，包括细胞粘附分子。本研究旨在确定镍钛合金丝（通过蚀刻和机械抛光或蚀刻和酸洗进行表面处理）与未处理丝相比释放的镍离子水平，并通过实时PCR在转录水平上确定丝对人脐静脉内皮细胞（HUVEC）的生物学影响。将四种不同的金属丝类型在培养基中孵育，并测定24、48和72小时后洗脱的镍量。然后培养HUVEC，并与四种不同的金属丝类型孵育24小时。收获细胞，分离RNA并进行实时PCR以测量与对照细胞相比的ICAM-1、VCAM-1和E-选择素（三种已知的炎症介质）的表达水平。E-选择素，内皮细胞损伤和活化的标志物，被发现在与释放最高量的镍离子的电线孵育的细胞中显著上调。镍离子从具有一定表面特性的镍钛合金丝中释放出来，这些离子对体外培养的人脐静脉内皮细胞具有生物学效应。
Nitinol has many applications in the medical device industry, however the large amount of nickel, a known allergen and carcinogen remains a serious concern. Studies have already shown that nickel ions induce the differential expression of a range of genes, including cell adhesion molecules. This study sought to determine the level of nickel ions released from nitinol wires that had been surface treated by etching and mechanically polishing or etching and pickling compared to untreated wires and determine the biological impact of the wires on human umbilical vein endothelial cells (HUVECs) at the transcriptional level by real-time PCR. The four different wire types were incubated in media and the amount of nickel eluted after 24, 48 and 72 h was determined. HUVECs were then cultured and incubated with the four different wire types for 24 h. Cells were harvested, RNA isolated and real-time PCR was carried out to measure the expression levels of ICAM-1, VCAM-1 and E-selectin, three known inflammatory mediators, compared to control cells. E-selectin, a marker of endothelial cell injury and activation was found to be significantly up-regulated in cells incubated with wires that released the highest amount of nickel ions. Nickel ions are released from nitinol wires with certain surface characteristics and these ions have a biological effect on HUVECs in vitro.