Analysis of Powdery Mildew Resistance in Wild Melon MLO Mutants
Analysis of Powdery Mildew Resistance in Wild Melon MLO Mutants
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野瓜MLO突变体的白粉病抗性分析
DOI：
10.16420/j.issn.2095-9885.2015-0036
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发表时间：
2015-11-01
影响因子：
5.7
通讯作者：
Li Jiping
中科院分区：
文献类型：
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作者：
Cheng Hong;Kong Weiping;Li Jiping
野生物种在作物育种中具有潜在的价值。发掘与甜瓜白粉病自然抗性相关的MLO基因，对甜瓜品质改良具有重要意义。用叶片接种法检测了栽培种和野生种对白粉病的抗性。野生种质对白粉病1号生理小种表现抗性。CmMLO2基因的克隆和序列分析表明，野生种和栽培种之间存在85个碱基的差异。荧光标记农杆菌介导法转化野生种质后，CmMLO2基因在野生种质中得到表达。转基因阳性植株表现出白粉病小种1的成功入侵。这些结果表明，野生种可能没有编码MLO蛋白，从而导致对白粉病的MLO负调控，进而导致野生种对白粉病的广谱抗性。
Wild species have a potential value in crop breeding. Explore MLO gene which related with powdery mildew natural resistance is very important for improving the quality of melon. Resistance to powdery mildew was examined in cultivar and wild species by leaf inoculation. The wild germplasms showed resistance to powdery mildew Race1. Cloning and sequence analysis of the CmMLO2 gene identified an 85 bp difference between the wild and cultivated species. The CmMLO2 gene was expressed in the wild germplasm after fluorescence-labeled Agrobacterium-mediated transformation. A positive transgenic plant showed successful invasion by powdery mildew Race1. These results suggested that the wild species might have failed to encode the MLO protein, thereby resulting in the MLO-negative regulation of powdery mildew, which in turn resulted in the broad-spectrum resistance of the wild species to powdery mildew.