Dichlorvos and lindane induced oxidative stress in rat brain: Protective effects of ginger
Dichlorvos and lindane induced oxidative stress in rat brain: Protective effects of ginger
复制标题
敌敌畏和林丹诱导大鼠大脑氧化应激：生姜的保护作用
作者：
Poonam Sharma;Rambir Singh
背景：敌敌畏和林丹农药会对包括人类在内的动物产生毒性。生姜（Zingiber officinale）在阿育吠陀医学体系中被广泛用作烹饪药物，具有多种药理特性。目的：本研究旨在评估姜汁对敌敌畏和林丹诱导的 Wistar 大鼠神经毒性的改善作用。材料和方法：对成年雄性和雌性 Wistar 大鼠单独或联合口服敌敌畏（8.8 毫克/千克体重）和林丹（8.8 毫克/千克体重）14 天，然后用姜汁（100 毫克/千克体重）后处理 14 天。测定脂质过氧化（LPO）、还原型谷胱甘肽（GSH）以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）、谷胱甘肽还原酶（GR）、奎宁还原酶（QR）等抗氧化酶的活性和蛋白质水平，以评估这些农药在脑中的毒性。结果：敌敌畏和林丹单独或联合使用可增加 LPO 并降低 GSH 水平、SOD、CAT、GPx、GST、GR、QR 活性和蛋白质。活性氧（ROS）异常产生导致的氧化应激被认为与这些农药引起的毒性有关。姜汁后处理可降低大鼠脑中 LPO 并增加 GSH、SOD、CAT、GPx、GST、GR、QR 活性和蛋白质水平。结论：结果表明，姜汁可改善敌敌畏和林丹引起的组织损伤。
Background: Dichlorvos and lindane pesticide causes toxicity in animals including humans. Ginger (Zingiber officinale) is widely used as a culinary medicine in the Ayurvedic system of medicine, possessing a number of pharmacological properties. Objective: This study was designed to assess ameliorating effects of ginger juice in dichlorvos and lindane induced neurotoxicity in wistar rats. Materials and Methods: Dichlorvos (8.8 mg/kg bw) and lindane (8.8 mg/kg bw) were orally administered alone as well as in combination to adult male and female wistar rats for 14 days followed by the post-treatment of ginger juice (100 mg/kg bw) for 14 days. Lipid peroxidation (LPO), reduced glutathione (GSH), and activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), quinine reductase (QR), and protein level were measured to evaluate the toxicity of these pesticides in brain. Results: Dichlorvos and lindane administration alone and in combination increased LPO and decreased the GSH level, SOD, CAT, GPx, GST, GR, QR activity, and protein. Oxidative stress due to abnormal production of reactive oxygen species (ROS) is believed to be involved in the toxicities induced by these pesticides. Post-treatment of ginger juice decreased LPO and increased the level of GSH, SOD, CAT, GPx, GST, GR, QR activity and protein in the brain of rats. Conclusions: The results indicated that dichlorovos and lindane induced tissue damage was ameliorated by ginger juice.