The venom of the spider Macrothele raveni induces apoptosis in the myelogenous leukemia K562 cell line
The venom of the spider Macrothele raveni induces apoptosis in the myelogenous leukemia K562 cell line
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拉氏大蜘蛛毒液诱导骨髓性白血病K562细胞系凋亡
DOI：
10.1016/j.leukres.2012.02.025
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发表时间：
2012-08-01
影响因子：
2.7
通讯作者：
Liang, Songping
中科院分区：
文献类型：
--
作者：
Liu, Zhonghua;Zhao, Yan;Liang, Songping
蜘蛛毒液是具有治疗潜力的生物活性化合物的丰富来源。在传统中医中，蜘蛛和蜘蛛毒液被用来治疗各种疾病。在本研究中，雷氏大蜘蛛毒液以剂量和时间依赖的方式抑制髓系白血病K562细胞的生长，其IC50为5.1微克/毫升。对人淋巴细胞也有较低的抑制作用，IC50约为36.4微克/毫升，表明该蛇毒对白血病细胞具有较强的选择性。蛇毒处理的K562细胞表现出典型的细胞凋亡的形态特征，包括核固缩和DNA片段化。Annexin V-FITC和碘化丙啶双重染色进一步证明该蛇毒具有较强的促凋亡活性。蛇毒处理可诱导K562细胞caspase3、caspase8活化，促进PARP裂解。本实验结果表明，雷氏蜘蛛毒液通过caspase3和caspase8介导的信号通路诱导K562细胞凋亡，从而有效、选择性地抑制K562细胞的生长。(C)2012爱思唯尔有限公司。保留所有权利。
Spider venoms are a rich source of bioactive compounds with therapeutic potential. In traditional Chinese medicine, spiders and spider venoms have been used in the treatment of various ailments. In the present study, the venom of the spider Macrothele raveni potently suppressed cell growth in the myelogenous leukemia K562 cell line in a dose and time-dependent manner with an IC50 of 5.1 mu g/mL. The venom also had a low inhibitory effect on human lymphocytes with an IC50 of approximately 36.4 mu g/mL, indicating that the venom is relatively selective for leukemic cells. Venom treated K562 cells showed typical morphological indicators of apoptosis including condensation of nuclei and fragmentation of DNA. Annexin V-FITC and propidium iodide dual staining further demonstrated that the venom had potent apoptogenic activity. Venom treatment induced caspase 3 and caspase 8 activation in K562 cells and promoted PARP cleavage. The present results indicate that the venom of the spider M. raveni potently and selectively suppresses the growth of K562 cells by inducing apoptosis via caspase 3 and caspase 8 mediated signaling pathways. (C) 2012 Elsevier Ltd. All rights reserved.