Genetic diversity of arsenic accumulation in rice and QTL analysis of methylated arsenic in rice grains.
Genetic diversity of arsenic accumulation in rice and QTL analysis of methylated arsenic in rice grains.
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DOI：
10.1186/1939-8433-6-3
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发表时间：
2013-01-11
期刊：
影响因子：
--
通讯作者：
Ishikawa S
中科院分区：
文献类型：
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作者：
Kuramata M;Abe T;Kawasaki A;Ebana K;Shibaya T;Yano M;Ishikawa S
对于以大米为主食的人群来说，大米是砷的主要膳食摄入来源。因此，有必要降低大米中砷的浓度，以避免对人体健康的潜在危害。本研究利用69份世界水稻核心材料（WRC），对水稻籽粒中砷积累和砷形成的遗传多样性进行了研究。此外，通过数量性状位点（QTL）分析，鉴定出控制水稻籽粒二甲基硅酸（DMA）含量的QTL。WRC籽粒As浓度差异达3倍。总砷、无机砷和DMA浓度受基因型、年份和基因型年互作效应的显著影响。在WRC品种中，本地巴斯马蒂和Tima（籼稻型）是稳定总砷和无机砷浓度最低的品种。利用来自Padi Perak（高DMA品种）和Koshihikari（低DMA品种）的F2群体，我们鉴定出6号染色体上的两个QTL （qDMAs6.1和qDMAs6.2）和8号染色体上的一个QTL （qDMAs8）与籽粒DMA浓度的变化有关。DMA约73%的总表型变异可由这三个qtl解释。根据上述结果，在低总砷含量的基础上改变有机砷的比例是培育低砷毒性水稻品种的策略之一。本文的在线版本（doi:10.1186/1939-8433-6-3）包含补充材料，可供授权用户使用。
Rice is a major source of dietary intake of arsenic (As) for the populations that consume rice as a staple food. Therefore, it is necessary to reduce the As concentration in rice to avoid the potential risk to human health. In this study, the genetic diversity in As accumulation and As speciation in rice grains was investigated using a world rice core collection (WRC) comprising 69 accessions grown over a 3-year period. Moreover, quantitative trait locus (QTL) analysis was conducted to identify QTLs controlling the dimethylarsinic acid (DMA) content of rice grains. There was a 3-fold difference in the grain As concentration of WRC. Concentrations of total-As, inorganic As, and DMA were significantly affected by genotype, year, and genotype-year interaction effects. Among the WRC accessions, Local Basmati and Tima (indica type) were identified as cultivars with the lowest stable total-As and inorganic As concentrations. Using an F2 population derived from Padi Perak (a high-DMA accession) and Koshihikari (a low-DMA cultivar), we identified two QTLs on chromosome 6 (qDMAs6.1 and qDMAs6.2) and one QTL on chromosome 8 (qDMAs8) that were responsible for variations in the grain DMA concentration. Approximately 73% of total phenotypic variance in DMA was explained by the three QTLs. Based on the results provided, one strategy for developing rice cultivars with a low level of toxic As would be to change the proportion of organic As on the basis of a low level of total As content. The online version of this article (doi:10.1186/1939-8433-6-3) contains supplementary material, which is available to authorized users.