Chip-based size-selective sorting of biological cells using high frequency acoustic excitation
Chip-based size-selective sorting of biological cells using high frequency acoustic excitation
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DOI：
10.1039/c1lc20050j
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发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Livermore, Carol
中科院分区：
文献类型：
--
作者：
Agarwal, Gunjan;Livermore, Carol
这项工作提出了单个生物细胞的大小选择性分选使用的组装过程称为模板组装选择性去除（TASR）。我们已经证明了实验，第一次，选择性放置和分选的单一SF 9细胞（克隆分离来自草地贪夜蛾（秋季粘虫）IPLB-Sf 21-AE细胞）到图案化的半球形网站刚性组装模板上使用TASR。在3分钟的短时间内，模板上几乎100%的组装位点被匹配细胞填充（组装密度高达900个位点/mm（2））。3-D重建的细胞轮廓和体积分析内的组装网站捕获的细胞表明，只有那些细胞，精确匹配的组装网站（0.5 μ m内）的大小组装在模板上。组装条件也与将TASR延伸至哺乳动物细胞相容。基于TASR的生物系统的尺寸选择性结构化和分选代表了一种有价值的工具，其具有在生物应用中实施的潜力，例如用于医学研究或诊断的细胞分选，用于人工组织复制的模板化，或用于生物学或机械行为研究的单细胞分离。
This work presents the size-selective sorting of single biological cells using the assembly process known as templated assembly by selective removal (TASR). We have demonstrated experimentally, for the first time, the selective placement and sorting of single SF9 cells (clonal isolate derived from Spodoptera frugiperda (Fall Armyworm) IPLB-Sf21-AE cells) into patterned hemispherical sites on rigid assembly templates using TASR. Nearly 100% of the assembly sites on the template were filled with matching cells (with assembly density as high as 900 sites per mm(2)) within short time spans of 3 minutes. 3-D reconstruction of cell profiles and volume analysis of cells trapped inside assembly sites demonstrates that only those cells that match the assembly site precisely (within 0.5 mu m) in size are assembled on the template. The assembly conditions are also compatible with the extension of TASR to mammalian cells. TASR-based size-selective structuring and sorting of biological systems represents a valuable tool with potential for implementation in biological applications such as cell sorting for medical research or diagnostics, templating for artificial tissue replication, or isolation of single cells for the study of biological or mechanical behavior.