Proper ascospore maturation requires the chs1+ chitin synthase gene in Schizosaccharomyces pombe
Proper ascospore maturation requires the chs1+ chitin synthase gene in Schizosaccharomyces pombe
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DOI：
10.1046/j.1365-2958.2000.01678.x
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发表时间：
2000-01-01
影响因子：
3.6
通讯作者：
Valdivieso, MH
中科院分区：
文献类型：
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作者：
Arellano, M;Cartagena-Lirola, H;Valdivieso, MH
我们已经克隆了chs 1（+），粟酒裂殖酵母基因与II类几丁质酶的相似性，并已表明，它是负责几丁质合成酶活性存在于细胞提取物从这种生物体。对这种活性的分析表明，它的行为类似于其他真菌的几丁质酶，尽管具有特定的生化特性。该基因的缺失或过表达不会导致营养生长过程中的任何明显缺陷。相反，chs 1（+）表达在孢子形成过程中显著增加，这伴随着几丁质合成酶活性的增加。此外，孢子形成受到严重影响时，两个亲本菌株进行chs 1缺失，作为一个结果，在子囊孢子细胞壁的合成缺陷。最后，我们表明，野生型，而不是chs 1（-）/chs 1（-），子囊孢子细胞壁结合麦胚凝集素。我们的研究结果清楚地表明，存在的关系chs 1（+），几丁质合成和子囊孢子成熟的S。粟酒
We have cloned chs1(+), a Schizosaccharomyces pombe gene with similarity to class II chitin synthases, and have shown that it is responsible for chitin synthase activity present in cell extracts from this organism. Analysis of this activity reveals that it behaves like chitin synthases from other fungi, although with specific biochemical characteristics. Deletion or overexpression of this gene does not lead to any apparent defect during vegetative growth. In contrast, chs1(+) expression increases significantly during sporulation, and this is accompanied by an increase in chitin synthase activity. In addition, spore formation is severely affected when both parental strains carry a chs1 deletion, as a result of a defect in the synthesis of the ascospore cell wall. Finally, we show that wild-type, but not chs1(-)/chs1(-), ascospore cell walls bind wheatgerm agglutinin. Our results clearly suggest the existence of a relationship between chs1(+), chitin synthesis and ascospore maturation in S. pombe.