Wnt-Responsive Stem Cell Fates in the Oral Mucosa
Wnt-Responsive Stem Cell Fates in the Oral Mucosa
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DOI：
10.1016/j.isci.2019.10.016
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发表时间：
2019-11-22
期刊：
影响因子：
5.8
通讯作者：
Helms, Jill A.
中科院分区：
文献类型：
--
作者：
Yuan, Xue;Xu, Quanchen;Helms, Jill A.
口腔上皮表现出形态和周转率的变化。这些差异与环境或区域特异性干细胞群有关吗？谱系追踪策略允许可视化wnt反应细胞及其后代，在硬腭和软腭。在这两个解剖部位，wnt反应性基底细胞自我更新并产生超基底细胞。腭部损伤引发了这个种群的扩大，它们的后代负责伤口的再上皮化。与硬腭相比，软腭干细胞表现出更早、更强劲的增殖爆发，最终明显更快地修复。此后，过量的wnt反应基底细胞被移除，干细胞数量恢复到稳态水平。因此，我们在口腔黏膜中发现了干细胞群，其相对丰度与口腔伤口愈合的速度相关。除了损伤时的激活外，修复后还存在一种内源性机制来约束干细胞池。
Epithelia of the oral cavity exhibit variations in morphologies and turnover rates. Are these differences related to environment or to region-specific stem cell populations? A lineage-tracing strategy allowed visualization of Wnt-responsive cells, and their progeny, in the hard and soft palates. In both anatomic locations, Wnt-responsive basal cells self-renewed and gave rise to supra-basal cells. Palatal injuries triggered an enlargement of this population, and their descendants were responsible for wound re-epithelialization. Compared with the hard palate, soft palate stem cells exhibited an earlier, more robust burst in proliferation, culminating in significantly faster repair. Thereafter, excess Wnt-responsive basal cells were removed, and stem cell numbers were restored back to homeostatic level. Thus, we uncovered a stem cell population in oral mucosa, and its relative abundance is correlate with the rate of oral wound healing. Besides the activation during injury, an endogenous mechanism exists to constrain the stem cell pool after repair.